Sample Instance

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{
    "data": {
        "type": "samples",
        "id": "ERS739170",
        "attributes": {
            "sample-metadata": [
                {
                    "key": "ENA checklist",
                    "value": "ERC000011",
                    "unit": null
                }
            ],
            "longitude": null,
            "biosample": "SAMEA3403120",
            "latitude": null,
            "accession": "ERS739170",
            "analysis-completed": null,
            "collection-date": null,
            "geo-loc-name": null,
            "sample-desc": "Protocols: intraperitoneal injection of anti-IL10 receptor antibody at days 0 and 7 For simultaneous DNA and RNA extraction, pellets were dissolved in 500 ul extraction buffer (200 mM NaCl, 20 mM EDTA, 4 M guanidine thiocyanate, 1 % (v/v) β-mercaptoethanol) and 210 ul 20 % (w/v) SDS and lysed with a bead beater twice for 2 minutes employing Lysing Matrix E tubes (MP Biomedicals). Samples were centrifuged at 8000 g for 5 minutes and the supernatant was diluted with 3 volumes of lysing buffer (ZR-Duet DNA/RNA Miniprep Kit, Zymo Research). DNA and RNA were further extracted employing the ZR-Duet DNA/RNA Miniprep Kit according to the manufacturer’s instructions. Total RNA was depleted of ribosomal RNA using Epibio’s universal bacteria ribo-zero kit, single version. Libraries were constructed using the NEBNext Ultra directional RNA Library Prep Kit (E7420L).",
            "environment-biome": null,
            "environment-feature": null,
            "environment-material": null,
            "sample-name": "E-MTAB-3562:rna_stool-aIL10R-R2",
            "sample-alias": "E-MTAB-3562:rna_stool-aIL10R-R2",
            "host-tax-id": null,
            "species": null,
            "last-update": "2023-03-04T06:56:12"
        },
        "relationships": {
            "runs": {
                "links": {
                    "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS739170/runs?format=api"
                }
            },
            "studies": {
                "links": {
                    "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS739170/studies?format=api"
                },
                "data": [
                    {
                        "type": "studies",
                        "id": "MGYS00006101",
                        "links": {
                            "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00006101?format=api"
                        }
                    }
                ]
            },
            "biome": {
                "data": {
                    "type": "biomes",
                    "id": "root:Host-associated:Mammals:Gastrointestinal tract:Intestine:Fecal"
                },
                "links": {
                    "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Host-associated:Mammals:Gastrointestinal%20tract:Intestine:Fecal?format=api"
                }
            }
        },
        "links": {
            "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS739170?format=api"
        }
    }
}