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{
"data": {
"type": "samples",
"id": "ERS712922",
"attributes": {
"latitude": 28.84,
"longitude": 153.6,
"biosample": "SAMEA3359426",
"sample-metadata": [
{
"key": "investigation type",
"value": "metagenome",
"unit": null
},
{
"key": "project name",
"value": "Metagenomic profiling of Dicathais orbita",
"unit": null
},
{
"key": "collection date",
"value": "2015-04",
"unit": null
},
{
"key": "sequencing method",
"value": "Roche 454",
"unit": null
},
{
"key": "NCBI sample classification",
"value": "69583",
"unit": null
},
{
"key": "instrument model",
"value": "454 GS FLX",
"unit": null
},
{
"key": "ENA checklist",
"value": "GSC MIxS host associated (ERC000013)",
"unit": null
},
{
"key": "host-associated environmental package",
"value": "host-associated",
"unit": null
}
],
"accession": "ERS712922",
"analysis-completed": "2017-02-15",
"collection-date": null,
"geo-loc-name": "Australia",
"sample-desc": "Dicathais orbita is a marine mollusc which is well known for the production of anticancer compounds that are precursors to Tyrian purple. This study aimed to assess the diversity and to identity bacteria associated with the Tyrian purple producing hypobranchial gland by comparison with foot tissues using a high-throughput sequencing approach. Total DNA was extracted from the hypobranchial gland and foot of D. orbita followed by high-throughput sequence, QIIME and MEGAN analysis of variable region V1-V3 of 16S rRNA bacterial gene amplicons. Metagenome analysis revealed a highly diverse bacterial assemblage associated with the hypobranchial gland and foot tissues of D. orbita. The dominant bacterial phylum in the metagenome data set was Proteobacteria followed by Tenericutes, Spirochaetes and Bacteroidetes. Larger numbers of bacterial taxa were present in the foot than the hypobranchial gland of D. orbita. This study revealed the high abundance of Vibrio spp. and the presence of unique bacterial communities in the hypobranchial gland which is also the site of indole production, bromoperoxidase activity and brominated secondary metabolites. This study also revealed the diversity of bacteria associated with the Tyrian Purple producing gland of a marine muricid and suggests bacteria have a potential role in the biosynthesis of Tyrian purple.",
"environment-biome": "coastal",
"environment-feature": "Intertidal rocky areas",
"environment-material": "water",
"sample-name": "Metagenomic profiling of bacterial communities associated with Tyrian Purple production in a marine gastropod",
"sample-alias": "Dicathais orbita1",
"host-tax-id": 69583,
"species": "Dicathais orbita",
"last-update": "2017-02-15T09:56:55"
},
"relationships": {
"biome": {
"data": {
"type": "biomes",
"id": "root:Host-associated:Mollusca:Digestive system:Glands"
},
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Host-associated:Mollusca:Digestive%20system:Glands?format=api"
}
},
"runs": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS712922/runs?format=api"
}
},
"studies": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS712922/studies?format=api"
},
"data": [
{
"type": "studies",
"id": "MGYS00001491",
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00001491?format=api"
}
}
]
}
},
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS712922?format=api"
}
}
}