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"last-update": "2017-02-13T14:49:33",
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"study-abstract": "Shifts in marine bacterial communities resulting from crude oil contamination were characterized by 16S rDNA PCR ? DGGE and 454 high-throughput sequencing with the aims to 1) reveal short-term changes in the structure of the marine bacterial community 2) identify the blooming bacteria resulting from oil occurrence by DNA analysis. A longer-term vision of this study is to use a selection of bacterial genes markers in combination with monitoring technology to sense and warn for oil contamination. Here, we present results obtained from seawater incubations with North Sea oil in absence (O) or presence (OD) of dispersant, carried out at low temperature (4 ?C) and in the presence of zooplankton. Despite the dispersant treatment, chemistry in O and OD was very similar possibly due to the setup with low mixing energy. Both O and OD treatments induced rapid (1 day) re-arrangement of bacterial community and enrichment of Pseudoalteromonas, Pseudomonas, Glaciecola, known as biosurfactant producers, as well as bacteria assigned to Sulfitobacter, Piscirickettsiaceae (Marine Methylotrophic Group 3), Neptunomonas and Vibrio. Pseudofulvibacter genus was enriched by oil in the treatment without dispersant. The oil- contaminated seawater contained bacterial genera of Polaribacter and Marinomonas identical in 98 -99 % to those found in the North Sea crude oil itself. This suggests that bacteria blooming in oil-contaminated seawater can also stem from the bacterial community in oil itself and contribute to the structural community shift observed.",
"study-name": "Capturing early changes in the marine bacterial community as a result of crude oil pollution in a mesocosm experiment",
"data-origination": "SUBMITTED"
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