GET /metagenomics/api/v1/samples/ERS427235/studies?format=api
HTTP 200 OK
Allow: GET, HEAD, OPTIONS
Content-Type: application/json
Vary: Accept
{
"links": {
"first": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS427235/studies?format=api&page=1",
"last": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS427235/studies?format=api&page=1",
"next": null,
"prev": null
},
"data": [
{
"type": "studies",
"id": "MGYS00000941",
"attributes": {
"samples-count": 67,
"accession": "MGYS00000941",
"bioproject": "PRJEB5969",
"is-private": false,
"last-update": "2016-05-16T09:56:40",
"secondary-accession": "ERP005417",
"centre-name": "CUSB-HM",
"public-release-date": null,
"study-abstract": "Animal models are invaluable tools which allow us to investigate the microbiome-host dialogue. However, experimental design introduces biases in the data that we collect, potentially leading to false conclusions. With obesity at pandemic levels animal systems of this disease have been developed; we investigated the role of experimental design on one such rodent model. We used 454 pyrosequencing to profile the faecal bacteria of obese (n = 6) and lean (homozygous n= 6; heterozygous n= 6) Zucker rats over a 10 week period, maintained in mixed-genotype cages, to further understand the relationships between the composition of the intestinal bacteria and age, obesity progression, genetic background and cage environment. Phylogenetic and taxon-based univariate and multivariate analyses (non-metric multidimensional scaling, principal component analysis) showed that age was the most significant source of variation in the composition of the faecal microbiota. Second to this, cage environment was found to clearly impact the composition of the faecal microbiota, with samples from animals from within the same cage showing high community structure concordance. Importantly, the obese phenotype was not found to impact the faecal bacterial profiles. These findings demonstrate that the age and local environmental cage variables were driving the composition of the faecal bacteria and were more deterministically important than host genotype. These findings have major implications for understanding the significance of functional metagenomic data in experimental studies and beg the question; what is being measured in animal experiments in which different strains are housed separately, nature or nurture?",
"study-name": "We analysed the gut microbiota of rats kept in the same cage",
"data-origination": "HARVESTED"
},
"relationships": {
"publications": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000941/publications?format=api"
}
},
"geocoordinates": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000941/geocoordinates?format=api"
}
},
"biomes": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000941/biomes?format=api"
},
"data": [
{
"type": "biomes",
"id": "root:Host-associated:Mammals:Digestive system",
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Host-associated:Mammals:Digestive%20system?format=api"
}
}
]
},
"downloads": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000941/downloads?format=api"
}
},
"samples": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000941/samples?format=api"
}
},
"analyses": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000941/analyses?format=api"
}
}
},
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000941?format=api"
}
}
],
"meta": {
"pagination": {
"page": 1,
"pages": 1,
"count": 1
}
}
}