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"id": "MGYS00006222",
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"bioproject": "PRJEB35687",
"samples-count": 76,
"accession": "MGYS00006222",
"is-private": false,
"last-update": "2023-07-18T11:38:12",
"secondary-accession": "ERP118779",
"centre-name": "Competence Center of Food and Fermentation Technologies",
"public-release-date": null,
"study-abstract": "OBJECTIVE The objectives were 1) determination of microbial composition of obese and normal weight subjects, 2) calculation of fiber composition of specially assigned low-calorie weight loss diet (WLD), 3) identification of microbial changes after WLD and improvements in health characteristics and associations of microbiota to fiber consumption profiles.METHODSA total of 19 overweight/obese participants with body mass index (BMI) of 28.9-44.4 kg/m2 were assigned to 20-40 % reduced calories high-fat low-carbohydrate diet for 4 weeks. Protein and fat content in composed diet was 1.5 times higher compared to that in average diet of normal weight group, while carbohydrate content was 2 times lower. Total fiber content was comparable to normal weight group. Food consumption data were obtained from assigned meals. Database of specific fiber composition of different food categories was developed and macronutrient, total and specific fiber consumption were calculated. Microbial composition was analysed before and after the intervention from 2 sequential samples by 16S rRNA sequencing. RESULTS:During intervention BMI reduced in average 2.5 ± 0.6 kg/m2 and stool frequency was normalized. Assigned diet induced significant changes in fecal microbiota. Abundance of bile resistant bacteria (Alistipes, Odoribacter splanchnicus), Ruminococcus bicirculans and Butyricimonas but also Enterobacteriaceae increased. Alistipes has been associated with lower BMI and Butyricimonas with higher values of HDL-cholesterol in blood. Importantly, abundance of bacteria often associated with inflammation such as Collinsella and Dorea decreased in parallel with decrease of BMI. Furthermore, the abundance of bifidobacteria decreased, which can be attributed to relatively low consumption of grains. CONCLUSIONS:In conclusion, results showed that short intervention studies can alter microbial community structure and gave relevant insights into development of different dietary concepts.",
"study-name": "Gut microbiome alterations after high-fat low-carbohydrate weight reduction diet: pros and cons",
"data-origination": "SUBMITTED"
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