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{
"data": {
"type": "samples",
"id": "ERS398664",
"attributes": {
"biosample": "SAMEA2340993",
"longitude": 10.1936,
"sample-metadata": [
{
"key": "investigation type",
"value": "metagenome",
"unit": null
},
{
"key": "experimental factor",
"value": "genetic variation, bacterial disease",
"unit": null
},
{
"key": "geographic location (longitude)",
"value": "10.193635",
"unit": null
},
{
"key": "geographic location (country and/or sea,region)",
"value": "Germany",
"unit": null
},
{
"key": "collection date",
"value": "2012-02-21",
"unit": null
},
{
"key": "environment (biome)",
"value": "organism-associated habitat",
"unit": null
},
{
"key": "environment (feature)",
"value": "organism-associated habitat",
"unit": null
},
{
"key": "environment (material)",
"value": "feces",
"unit": null
},
{
"key": "environmental package",
"value": "host-associated",
"unit": null
},
{
"key": "target gene",
"value": "16S rRNA",
"unit": null
},
{
"key": "sequencing method",
"value": "pyrosequencing",
"unit": null
},
{
"key": "geographic location (latitude)",
"value": "53.82115",
"unit": null
},
{
"key": "instrument model",
"value": "454 GS FLX Titanium",
"unit": null
},
{
"key": "host disease status",
"value": "Healthy",
"unit": null
},
{
"key": "host genotype",
"value": "\"endothel= +; epithelia= -/-\"",
"unit": null
}
],
"latitude": 53.8212,
"accession": "ERS398664",
"analysis-completed": "2017-04-28",
"collection-date": "2012-02-21",
"geo-loc-name": "Germany",
"sample-desc": "Glycans on mucosal surfaces play an important role in host-microbe interactions. Expression of the blood group-related glycosyltransferase ?-1,4-N-acetylgalactosaminyltransferase 2 (B4galnt2) is variable in wild mouse populations and influences the composition of the intestinal microbiota. In this study we show that the presence of B4galnt2 glycans in the intestinal epithelium increases susceptibility to Salmonella Typhimurium infection, leading to increased levels of pro-inflammatory cytokines and higher numbers of infiltrating immune cells. Further, changes in the intestinal microbiota correlated significantly with the development of inflammation and the associated B4galnt2 genotype. Specifically, the higher phylogenetic diversity of the intestinal microbiota present in B4galnt2-deficient mice before infection was predictive for a reduced inflammatory response to Salmonella Typhimurium. These data support the hypothesis that variation in B4galnt2 expression and associated microbial patterns alters host susceptibility to infectious diseases of the gastrointestinal tract and may explain the patterns of long term balancing selection observed in wild mouse populations.",
"environment-biome": "organism-associated habitat",
"environment-feature": "organism-associated habitat",
"environment-material": "feces",
"sample-name": "GG04_14_d-1",
"sample-alias": "GG04_14_d-1",
"host-tax-id": 10090,
"species": "Mus musculus",
"last-update": "2017-04-28T16:47:22"
},
"relationships": {
"biome": {
"data": {
"type": "biomes",
"id": "root:Host-associated:Mammals:Digestive system:Large intestine:Fecal"
},
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Host-associated:Mammals:Digestive%20system:Large%20intestine:Fecal?format=api"
}
},
"studies": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS398664/studies?format=api"
},
"data": [
{
"type": "studies",
"id": "MGYS00001721",
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00001721?format=api"
}
}
]
},
"runs": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS398664/runs?format=api"
}
}
},
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS398664?format=api"
}
}
}