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{
"data": {
"type": "samples",
"id": "ERS311679",
"attributes": {
"longitude": -71.0932,
"latitude": 42.3591,
"biosample": "SAMEA2169236",
"sample-metadata": [
{
"key": "sex",
"value": "female",
"unit": null
},
{
"key": "investigation type",
"value": "mimarks-survey",
"unit": null
},
{
"key": "project name",
"value": "Molecular Determinants of Helicobacter hepaticus pathogenesis",
"unit": null
},
{
"key": "collection date",
"value": "10.12.2009",
"unit": null
},
{
"key": "environmental package",
"value": "host-associated",
"unit": null
},
{
"key": "target gene",
"value": "16S rDNA",
"unit": null
},
{
"key": "target subfragment",
"value": "V1 - V2",
"unit": null
},
{
"key": "pcr primers",
"value": "8F (5'-AGAGTTTGATCCTGGCTCAG-3'), 338R (5'-TGCTGCCTCCCGTAGGAGT-3'); sequencing primer: 338R",
"unit": null
},
{
"key": "multiplex identifiers",
"value": "AGACGCACTC",
"unit": null
},
{
"key": "adapters",
"value": "5'-GCCTTGCCAGCCCGCTCAG-multiplex_idendifier-CA; 5'-GCCTTGCCAGCCCGCTCAGTC-3'",
"unit": null
},
{
"key": "pcr conditions",
"value": "50 µl reaction containing 30-50 ng DNA, 0.3 µM of each primer, 2.5 mM Mg2+, 0.2 mM of each dNTP, 2.5 U HotMaster Taq Polymerase (5PRIME). Initial denaturation: 95degC_ 2 min; denaturation: 95degC_20 s; annealing: 52degC_20s; elongation: 65degC_1min; 30 cycles.",
"unit": null
},
{
"key": "sequencing method",
"value": "pyrosequencing",
"unit": null
},
{
"key": "age",
"value": "172",
"unit": "days"
},
{
"key": "body site",
"value": "cecum",
"unit": null
},
{
"key": "genotype",
"value": "Il10-/-",
"unit": null
},
{
"key": "host common name",
"value": "mouse",
"unit": null
},
{
"key": "host taxid",
"value": "10090",
"unit": null
},
{
"key": "infra_specific_rank",
"value": "C57BL/6J",
"unit": null
},
{
"key": "body habitat",
"value": "cecum",
"unit": null
},
{
"key": "NCBI sample classification",
"value": "410661",
"unit": null
},
{
"key": "instrument model",
"value": "454 GS FLX",
"unit": null
},
{
"key": "ENA checklist",
"value": "GSC MIxS host associated (ERC000013),GSC MIxS host associated (ERC000013)",
"unit": null
},
{
"key": "ENA-SUBMISSION-TOOL",
"value": "SRA-Webin",
"unit": null
}
],
"accession": "ERS311679",
"analysis-completed": "2016-09-28",
"collection-date": null,
"geo-loc-name": "USA",
"sample-desc": "Intestinal microbiota of interleukin-10 deficient C57BL/6J mice.",
"environment-biome": "Animal facility",
"environment-feature": "specific pathogen-free",
"environment-material": "air",
"sample-name": "Intestinal microbiota of interleukin-10 deficient C57BL/6J mice.",
"sample-alias": "MIT cecum mouse 3",
"host-tax-id": 10090,
"species": "Mus musculus",
"last-update": "2016-09-28T09:43:40"
},
"relationships": {
"biome": {
"data": {
"type": "biomes",
"id": "root:Host-associated:Mammals:Digestive system:Large intestine:Fecal"
},
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Host-associated:Mammals:Digestive%20system:Large%20intestine:Fecal?format=api"
}
},
"studies": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS311679/studies?format=api"
},
"data": [
{
"type": "studies",
"id": "MGYS00001233",
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00001233?format=api"
}
}
]
},
"runs": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS311679/runs?format=api"
}
}
},
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS311679?format=api"
}
}
}