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"id": "MGYS00002377",
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"bioproject": "PRJEB26649",
"samples-count": 192,
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"last-update": "2018-05-21T12:01:13",
"secondary-accession": "ERP108656",
"centre-name": "EAWAG",
"public-release-date": null,
"study-abstract": "Rivers receive a wide range of micro-pollution from wastewater treatment plants (WWTPs). WWTP effluent adds nutrients and chemicals into streams and follows well-documented hydrological mixing patterns based on dilution. However, the hydrological mixing of planktonic microbial communities is less well-documented. We tested whether environmental DNA (eDNA) sampled from 24 headwater streams and WWTP effluent flowing into the economically important Rhine River followed predicted dilution patterns and characterized the bacterial communities using 16S rRNA amplicon sequencing. Total eDNA recovered from the effluent lacked a quantitative mixing relationship with receiving streams, suggesting that eDNA is influenced by more than hydrological dilution. The downstream 16S profiles highlighted a decrease in Cyanobacteria and Proteobacteria taxa and an increase in Firmicutes and TM7 taxa compared to the upstream. All resulting sequences were assigned in silico habitat descriptors, and these habitat descriptors matched expected values for the effluent (e.g., “wastewater”) and upstream (e.g., “freshwater lake”, “river”) for most streams, whereas the downstream habitat descriptors were a mixture between the two. Thus, we demonstrate that effluent alters water column bacterial communities in receiving streams. This qualitative alteration of bacterial communities warrants further investigation into how bacterial pollution from WWTPs affects ecosystem function and stress.",
"study-name": "Environmental DNA profiles capture the influence of treated wastewater effluent on water column bacterial composition in headwaters of the Rhine River",
"data-origination": "SUBMITTED"
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