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            "id": "MGYS00005720",
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                "bioproject": "PRJEB25224",
                "accession": "MGYS00005720",
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                "last-update": "2021-03-17T14:26:54",
                "secondary-accession": "ERP107119",
                "centre-name": "INSTITUT DE CIENCIES DEL MAR",
                "public-release-date": null,
                "study-abstract": "Surface global ocean prokaryotic picoplankton from the Malaspina-2010 expedition: Surface waters (3m depth) from a total of 122 globally-distributed stations located in the tropical and sub-tropical global ocean were sampled from December 2010 to July 2011 as part of the Malaspina-2010 expedition (Duarte 2015). Water samples were obtained with Niskin bottles attached to a CTD profiler that included sensors for conductivity, temperature, oxygen, fluorescence and turbidity. About 12L of seawater were sequentially filtered through a 20m nylon mesh, followed by a 3m and 0.2m polycarbonate filters of 47mm diameter. Only the picoplankton size-fraction (0.2-3 m) was used for sequencing. DNA was extracted using a standard phenol-chloroform protocol (Massana, et al. 1997). The V4-V5 region of the 16S rRNA gene was amplified with the primers 515F-Y (5'-GTGYCAGCMGCCGCGGTAA) and 926R (5'-CCGYCAATTYMTTTRAGTTT)  (Parada et al. 2016) and sequenced in an Illumina MiSeq platform (2x250bp) at the Research and Testing Laboratory  (Lubbock, Texas, USA; http://www.researchandtesting.com).\n\nDuarte CM. 2015. Seafaring in the 21St Century: The Malaspina 2010 Circumnavigation Expedition. Limnology and Oceanography Bulletin 24:11-14.\n\nMassana R, Murray AE, Preston CM, DeLong EF. 1997. Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel. Applied and environmental microbiology 63:50-56.\n\nParada AE, Needham DM, Fuhrman JA. 2016 Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environ Microbiol. 18: 1403-14.",
                "study-name": "Picoplankton 16S rRNA genes from the tropical and sub-tropical global-ocean sampled during the Malaspina-2010 expedition",
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