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{
"data": {
"type": "samples",
"id": "ERS167167",
"attributes": {
"longitude": -0.3775,
"biosample": "SAMEA2164663",
"latitude": 39.4697,
"sample-metadata": [
{
"key": "sample storage temperature",
"value": "-80",
"unit": "°C"
},
{
"key": "sex",
"value": "male",
"unit": null
},
{
"key": "geographic location (longitude)",
"value": "-0.3775",
"unit": null
},
{
"key": "geographic location (country and/or sea,region)",
"value": "Spain",
"unit": null
},
{
"key": "collection date",
"value": "29/07/2010",
"unit": null
},
{
"key": "environment (biome)",
"value": "terrestrial biome ENVO:00000446",
"unit": null
},
{
"key": "environment (feature)",
"value": "human-associated habitat",
"unit": null
},
{
"key": "environment (material)",
"value": "feces",
"unit": null
},
{
"key": "environmental package",
"value": "human-gut",
"unit": null
},
{
"key": "sample collection device or method",
"value": "Feces were self-collected by the volunteers in tubes containing 10 mL of phosphate buffered saline (PBS). Samples were kept at 4 �C until their storage at -80 �C (approximately 2 hours after being collected)",
"unit": null
},
{
"key": "sample material processing",
"value": "Before nucleic acid extraction, fecal samples were resuspended in PBS and centrifuged at 4,000 rpm for 3 min to remove fecal debris. 1-4 mL of the supernatants were centrifuged at 14,000 rpm for 3 min to recover cell pellets",
"unit": null
},
{
"key": "nucleic acid extraction",
"value": "DNA and RNA were simultaneously extracted from cell pellets using the AllPrep DNA/RNA Mini Kit (QIAGEN)",
"unit": null
},
{
"key": "age",
"value": "66",
"unit": null
},
{
"key": "body product",
"value": "feces",
"unit": null
},
{
"key": "body-mass index",
"value": "31.7",
"unit": null
},
{
"key": "diet",
"value": "mediterranean diet",
"unit": null
},
{
"key": "disease status",
"value": "not applicable",
"unit": null
},
{
"key": "family relationship",
"value": "husband of P1",
"unit": null
},
{
"key": "host common name",
"value": "Homo sapiens",
"unit": null
},
{
"key": "IHMC ethnicity",
"value": "caucasian",
"unit": null
},
{
"key": "geographic location (latitude)",
"value": "39.46972222",
"unit": null
},
{
"key": "gastrointestinal tract disorder",
"value": "none",
"unit": null
},
{
"key": "smoker",
"value": "ex-smoker",
"unit": null
},
{
"key": "GOLD sample classification",
"value": "Host-associated > Human > Digestive system > Large intestine > Fecal",
"unit": null
},
{
"key": "NCBI sample classification",
"value": "408170",
"unit": null
},
{
"key": "instrument model",
"value": "454 GS FLX Titanium",
"unit": null
}
],
"accession": "ERS167167",
"analysis-completed": "2012-08-29",
"collection-date": "2010-07-29",
"geo-loc-name": "Spain; Valencia",
"sample-desc": "fecal sample of Control 1 on day 37 of the follow-up",
"environment-biome": "terrestrial biome ENVO:00000446",
"environment-feature": "human-associated habitat",
"environment-material": "feces",
"sample-name": "Control 1 Day 37",
"sample-alias": "C1-D37",
"host-tax-id": 9606,
"species": "Homo sapiens",
"last-update": "2015-08-13T15:30:55"
},
"relationships": {
"studies": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS167167/studies?format=api"
},
"data": [
{
"type": "studies",
"id": "MGYS00000308",
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000308?format=api"
}
}
]
},
"runs": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS167167/runs?format=api"
}
},
"biome": {
"data": {
"type": "biomes",
"id": "root:Host-associated:Human:Digestive system:Large intestine:Fecal"
},
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Host-associated:Human:Digestive%20system:Large%20intestine:Fecal?format=api"
}
}
},
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS167167?format=api"
}
}
}