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"bioproject": "PRJEB58159",
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"last-update": "2023-07-18T10:32:19",
"secondary-accession": "ERP143222",
"centre-name": "ROSWELL PARK CANCER INSTITUTE",
"public-release-date": null,
"study-abstract": "Briefly, male, 2-3-month old, C57BL/6J mice were treated with high-fat diet for 16 weeks to induce obesity. Age/sex-matched control mice were fed normal diet. DNA was isolated from lungs after sacrificing mice using Qiagen® DNeasy PowerSoil Kit. 16S PCR and library preparation were as per Illumina® Metagenomics Guide, using the guide's suggested V3-V4 primers; Kapa Biosystems® KAPA HiFi ReadyMix for PCR, with 35 cycles and 25 ng DNA input (when available; otherwise entire DNA sample). Indexing was with Nextera® XT v2 combinatorial dual index kit, 8 cycles of PCR. Negative controls used were for DNA extraction (three) and 16S PCR (no template; two). One positive control was ZymoBIOMICSTM Microbial Community DNA Standard, Zymo® cat. #6305). All libraries were sequenced together, at ~8 pM each, with 10% PhiX library spike-in, on Illumina® MiSeq with v3 reagents, to obtain 300 b paired-end reads.",
"study-name": "Bacterial microbiomes of lungs of male C57BL/6J mice optionally treated with high-fat diet to induce obesity",
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