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"bioproject": "PRJEB13056",
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"last-update": "2023-07-18T11:26:40",
"secondary-accession": "ERP014594",
"centre-name": "Academic Medical Center, University of Amsterdam",
"public-release-date": null,
"study-abstract": "Rationale: Mechanical ventilation is assumed to cause a shift in pulmonary microbial communities. Ventilator-associated pneumonia (VAP) is a frequent complication of prolonged mechanical ventilation and has a considerable morbidity and mortality. In this study we hypothesized that microbial diversity lowers with prolonged mechanical ventilation and as antibiotics are administrated; there was an effect of dominance of a subset of well-adapted bacterial pathogens and these selection was more profound in patients that developed VAP. Methods: This is a case–control longitudinal study of patients that were originally included in an international multi–centre prospective observational cohort study of the predictive value of biological markers for development of VAP. Repeated sampling approach was applied during mechanical ventilation. Total DNA was extracted from endotracheal aspirates and variable regions 2 and 3 of bacterial 16S rRNA gene were amplified and sequenced. Results: Antibiotic use was not associated with bacterial biodiversity decrease. There was an increase in relative abundance of Pseudomonadales, Enterobacteriales, Bacillales, and Burkholderiales within mechanical ventilation period, however it was not correlated with VAP development. In the microbial network potential pathogens Acinetobacter, Pseudomonas, Staphylococcus, Haemophilus tend to mutually exclude other species. Conclusion: Repeated sampling approach is a useful tool to study respiratory microbiome. Together with characterization of host immune factors it will improve our understanding of microbiome dysbiosis caused by mechanical ventilation.",
"study-name": "The dynamics of the pulmonary microbiome during mechanical ventilation in the intensive care unit and the association with occurrence of pneumonia.",
"data-origination": "SUBMITTED"
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