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"study-abstract": "The Third Party Annotation (TPA) assembly was derived from the primary whole genome shotgun (WGS) data set PRJEB52452, and was assembled with metaspades v3.15.3. This project includes samples from the following biomes: root:Environmental:Aquatic:Marine.",
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"study-abstract": "Here we present a dataset of 76 microbial metagenomes of the picoplankton size fraction (0.2-3.0 μm) collected in 11 stations along the Malaspina Expedition circumnavigation (http://www.expedicionmalaspina.es; Duarte 2015) that cover vertical profiles sampled at 7 depths, from the surface to 4,000 m deep (or the sea floor in shallower waters), plus 5 additional metagenomes. This Malaspina Microbial Vertical Profiles metagenomes (MProfile) dataset produced 1.66 Tbp of raw DNA sequences. Six L seawater samples were collected and filtered through a 200 and a 20 μm mesh to remove large plankton, the prokarotic free-living size fraction (0.2 to 3.0 μm) was recovered in each sample by pumping water serially through 47-mm polycarbonate membrane filters of 3.0 μm and 0.22 μm pore sizes with a peristaltic pump. DNA was extracted using a standard phenol-chloroform protocol and was sequenced at the National Center for Genomic Analsis (CNAG-CRG. Barcelona, Spain; www.cnag.es), funded by project MALASPINOMICS (CTM2011-15461-E, led by Carlos M Duarte) on the Illumina HiSeq2000 sequencing platform utilizing a TruSeq paired-end cluster kit, v3, following a 2x100 indexed run recipe. \n\nReferences: Duarte CM. 2015. Seafaring in the 21St Century: The Malaspina 2010 Circumnavigation Expedition. Limnology and Oceanography Bulletin 24:11-14; Sánchez, P. et al., 2023. Marine picoplankton metagenomes from eleven vertical profiles obtained by the Malaspina Expedition in the tropical and subtropical oceans. bioRxiv 2023.02.06.526790; doi: https://doi.org/10.1101/2023.02.06.526790",
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