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"study-abstract": "A significant proportion of the marine prokaryote community is comprised of microbes that utilise proteorhodopsin (PR), a retinal based photoreceptor used for generating ATP from light. Using an amplicon sequencing approach, we examined spatial and temporal patterns in the diversity of PR containing Bacteria and Archaea (PRBA) along the east coast of Australia, during a period of 12 month from surface samples collected at 3 oceanographic time-series sites spanning 15° of latitude. Coastal PRBA assemblages were dynamic in both space and time, with diversity linked to several environmental factors, with positive correlations to temperature, Secchi depth and Prochlorococcus cell abundance and negative correlations to phosphate concentration observed. Shifts in the taxonomic composition of the PRBA assemblage were best explained by temperature and day length. Seasonality in taxonomic structure was accompanied by temporal variability in proteorhodopsin spectral tuning characteristics. The ratio of blue absorbing proteorhodopsin proteins (BPR) to green absorbing proteorhodopsin proteins (GPR) was strongly linearly correlated with day length, with levels of GPR increasing with day length. These observations highlight the dynamic nature of proteorhodopsin containing marine assemblages, which exhibit substantial shifts in both taxonomy and spectral tuning properties in response to seasonal and spatial variability in environmental conditions.",
"study-name": "Regional heterogeneity and seasonal succession in proteorhodopsin containing marine microbial assemblages",
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"id": "MGYS00000615",
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"bioproject": "PRJEB12796",
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"last-update": "2016-04-19T00:05:03",
"secondary-accession": "ERP014305",
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"study-abstract": "Observational and modelling studies have shown that currents along the east coast boundary of Tasmania are influenced by the strength of a residual East Australian current (EAC), experiencing distinctive seasonal and interannual variation in water properties. In this study we investigated whether the influence of the EAC changes the microbial community structure, including photoheterotrophic proteorhodopsin-containing bacteria (PRB), a key component of the microbial community. Seawater samples were collected from August 2011 to March 2012 from surface waters at the National Reference Station Maria Island Mooring. The microbial communities were characterized by 454 pyrosequencing of the 16S rRNA and proteorhodopsin genes. Our data show marked changes in microbial assemblages over the season, in particular in February and March. The maximal effect of the EAC was observed by the increased abundance of Prochlorococcus spp., associated with warm and highly oligotrophic open ocean waters. PRB sequence diversity indicated that Alphaproteobacteria related clades SAR116 and SAR11 predominated at most times while Archaea marine group II predominated mostly in winter. Quantitative PCR demonstrated that SAR 11 PRB populations were more abundant during the last months of summer and were positively correlated with pigments (i.e. divinyl chlorophyll a and zeaxanthin), and inversely correlated to nutrient (NO2-/NO3- and PO43-) concentrations. Overall, these findings indicate that further increases in the influence of the EAC could alter the bacterioplankton community structure towards that of typical of open ocean systems.",
"study-name": "454Titanium barcoded pyrosequencing of the 16S rRNA and proteorhodopsin-containing photoheterotrophs genes was used to characterize the structure and microbial diversity across a seasonal change (August to March), in waters off Maria Island.",
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