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"accession": "MGYS00001730",
"is-private": false,
"last-update": "2020-05-20T21:30:49",
"secondary-accession": "ERP006234",
"centre-name": "UNIV-WUE",
"public-release-date": null,
"study-abstract": "Culture-dependent surveys of the oral cavity of snakes have found a wide array of potential human pathogens, hence bacterial infections secondary to snakebites and cases of salmonellosis have been linked to the oral microbiota of reptiles. Further, another set of literature records demonstrate that reptile oral bacteria are attributable to prey feces. Here, we used culture-independent16S rDNA amplicon sequencing to characterize the oral microbiota of snakes and other common pet reptiles and to test for the proportion of bacteria reported in literature. In our oral samples the vast majority of reported bacteria were either absent or present in very low proportions. We found strong differences between the taxonomic groups of reptiles, suggesting a highly specific and unique microbiota structure. Moreover, the oral microbiome of snakes was very different from the fecal microbiota reported for mice. Our study provides a first comprehensive survey with next-generation sequencing investigating the oral microbiota of snakes and reptiles in general and points out that it is important to readdress the current knowledge with culture independent techniques.",
"study-name": "Culture-independent assessment of oral microbiota composition in pet reptiles",
"data-origination": "SUBMITTED"
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"id": "root:Host-associated:Reptile:Oral cavity",
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{
"type": "studies",
"id": "MGYS00004557",
"attributes": {
"samples-count": 100,
"bioproject": "PRJEB15058",
"accession": "MGYS00004557",
"is-private": false,
"last-update": "2019-04-12T12:53:09",
"secondary-accession": "ERP016749",
"centre-name": "UCSDMI",
"public-release-date": null,
"study-abstract": "These samples represent the hindgut swabbings of 76 Green Iguanas (66 hatchlings, three subadults, seven adults), with hatchlings from nine sites (Gamboa, Panama and eight sites on or around Barro Colorado Island, Panama). We included an equal number of hatchlings from each site when possible. Hatchling social behavior (i.e., size of group, proximity to others) was observed haphazardly for individuals for about 60 days post-emergence over two years. (Adults and subadults were sampled and included whenever possible.) We primarily included samples taken about 4-6 weeks into the season for direct comparisons across sites and social levels. We have multiple samples (mean 3 per lizard) for 12 hatchlings spanning several days to several weeks between samples.",
"study-name": "Intergenerational Lizard Lounges do not Explain Variation in the Gut Microbiomes of Green Iguanas",
"data-origination": "SUBMITTED"
},
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{
"type": "studies",
"id": "MGYS00001458",
"attributes": {
"samples-count": 60,
"bioproject": "PRJEB4882",
"accession": "MGYS00001458",
"is-private": false,
"last-update": "2017-02-13T13:44:57",
"secondary-accession": "ERP004214",
"centre-name": "EBI-UI",
"public-release-date": null,
"study-abstract": "While much has been known about the fecal microbiome of mammalian herbivorous, little is known about the fecal microbiome of reptilian herbivores. To elucidate the functional gene diversity of reptilian herbivores, we performed a metagenome-based analysis of the fecal samples from the algae-consuming marine iguana (Amblyrhynchus cristatus) and terrestrial flora-consuming land iguanas (genus Conolophus) that are indigenous on the Gal?pagos Archipelago. Further comparison was made against the mammalian herbivorous hosts, and our findings suggested that the phylogenetic affiliations of both land iguanas (LI) and marine iguanas (MI) fecal microbiota clustered apart, and shared low similarity with the mammalian herbivorous hosts. Furthermore, functional gene diversities in both iguana hosts showed differentiation based on the diet, in which the MI fecal microbiota had a functional diversity that clustered apart from the other terrestrial-flora consuming LI iguana and herbivorous mammalian hosts. The differentiation in the functional gene diversity of MI fecal microbiota is likely attributed to a distinctly unique diet that is based on marine algae. A further examination of the carbohydrate-degrading genes revealed that several of the prevalent glycosyl hydrolases (GH), glycosyl transferases (GT), carbohydrate binding modules (CBM) and carbohydrate esterases (CE) gene classes were conserved among all examined herbivorous mammalian and reptilian hosts, reiterating the important roles these genes have in the breakdown and metabolism of herbivorous diet. However, some classes of carbohydrates-degrading families like GH2, GH13, GT2, GT4, CBM50, CBM48, CE4 and CE11, as well as genes associated with sulfur metabolism and dehalogenation were highly expressed or unique to the MI fecal microbiota. In contrast, gene sequences that relate to archaeal methanogenesis were detected only in LI fecal microbiome, and carbohydrate-degrading families like GH13, GH66, GT2, GT4, CBM50, CBM13, CE4 and CE8 were highly abundant in the LI. Certain bacterial populations were enriched over ten generations in various substrates (e.g. glucose, arabinose, xylose), and the enriched populations exhibited differences based on the iguana host and the type of enrichment substrate. Majority of the enriched bacterial populations belong to genera Clostridium spp. and Enterococcus spp. that likely accounted for the high prevalence of GH13 and GH2, as well as the GT families (e.g. GT2, GT4, GT28, GT35 and GT51) that were ubiquitously present in the fecal microbiota of all herbivorous hosts.",
"study-name": "This study utilizes a metagenome-based approach to elucidate the phylogenetic and functional gene diversity of fecal microbiome in Galapagos land and marine iguanas.",
"data-origination": "SUBMITTED"
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