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                "study-abstract": "The aim of this study is to assess the link between gut microbiota composition and subsequent occurrence of ventilator-associated pneumonia in critically ill influenza patients. Every patient admitted to intensive care unit from October 2019 to March 2020 who received at least 2 days of invasive mechanical ventilation were included (ancillary study of Microbe study, NCT04131569). Rectal swabs were sampled and DNA extraction was performed by QIAamp® PowerFecal® Pro DNA kit (QIAgen®). V3-V4 regions of 16SRNA coding gene was amplified to analyse the bacterial kingdom of lung microbiota (bacteriobiota) and ITS2 to analyse the fungal kingdom (mycobiota). Sequencing (2x250bp paired-end reads) was performed on MiSeq sequencer (Illumina®) and DADA2 pipeline on R software was used for bioinformatics analyses.",
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            "id": "MGYS00006004",
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                "last-update": "2023-03-03T19:10:06",
                "secondary-accession": "ERP134914",
                "centre-name": "Latvian Biomedical Research and Study Centre",
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                "study-abstract": "The gut microbiota and their products have a critical role in human health and are involved in all physiological processes. It is well now known that altered microflora can lead to the development of various diseases, including malignancies. One of the ways in which these processes in the body are potentially influenced by the microflora is through extracellular vesicles (EVs) and the RNA content in them, but currently research methods for these processes are limited. Classical in vitro methods cannot mimic the entry of the microbiome EV from the intestinal lumen into the circulatory system, but animal models do not fully reflect the processes in the human body and are ethically questionable models for these studies. One of the most promising methods currently in microflora research is the gut on chip (GoC) platform, however so far, the available solution has not been applied and designed for the study of the microbiome EV content. Therefore, the aim of this project is to study EV RNA content of cancer patient microbiome, that can enter from lumen to circulation by applying GoC devices already developed in a collaboration between BMC and ISSP. These results will provide answers to fundamental questions about human-microorganism interactions in the future, as well as give the opportunity to study and testing of clinical microflora samples from different patients for diagnostic and therapeutic purposes.",
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                "bioproject": "PRJEB13171",
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                "last-update": "2016-04-15T10:01:39",
                "secondary-accession": "ERP014712",
                "centre-name": "URMITE",
                "public-release-date": null,
                "study-abstract": "The study of the human gut microbiota was relaunched by metagenomic studies.",
                "study-name": "Gut metagenomes from culturomics",
                "data-origination": "SUBMITTED"
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