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"study-abstract": "We report 16S rRNA gene amplicon sequence analysis of gut microbiota of deep-sea fishes (Chlorophthalmus albatrossis, Glossanodon semifasciatus, and Helicolenus hilgendorfi) collected from Suruga Bay, Japan. The analysis revealed a low taxonomic diversity of gut microbiota in these deep-sea fishes.",
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"study-abstract": "Fish intestinal mycobiota are hitherto poorly characterized. Using next generation sequencing, we have profiled the intestinal mycobiota of wild caught, laboratory-reared and wild-caught-laboratory-kept zebrafish, which contained more than 15 fungal classes. In wild zebrafish, mycobiota comprised mainly Dothideomycetes, whereas saprotrophic Saccharomycetes were predominant in their laboratory-reared counterparts. This pioneer study shed light into differences in intestinal fungal communities of wild-caught and laboratory-reared zebrafish, thus enriching our knowledge on fish mycobiota.",
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"secondary-accession": "SRP115243",
"centre-name": "Edith Cowan University",
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"study-abstract": "Globally, marine species distributions are being modified as a result of rising sea surface temperature. On the west coast of Australia, the southern distributional limits of several tropical herbivorous fish species, including the rabbitfish Siganus fuscescens, have recently expanded into temperate regions. Microbes are fundamentally important to animal health, demanding an understanding of their variation in studies of animal adaption. Range-shifting S. fuscescens thus provide a unique opportunity to assess the stability of gastrointestinal microbes under varying environmental conditions. Here, the gastrointestinal microbial communities of S. fuscescens were characterised over 2000km of Western Australia's coastline, including in the species' historical and current southern range limit. MiSeq Illumina sequencing of the 16S rRNA gene demonstrated that the microbial community differed among populations, and there was a general decrease in hindgut microbial community similarity with distance. However, the population in the newly expanded range had similar hindgut microbial communities to one population in the historical range and levels of short chain fatty acids, an indicator of microbial fermentation activity, were similar among tropical and temperate locations. These data suggest that flexibility in the hindgut microbiome may play a role in enabling range-shifting herbivores to colonise new habitats.",
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"id": "MGYS00002608",
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"secondary-accession": "ERP110004",
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"study-abstract": "Probiotics are live microbial strains incorporated as dietary supplements in feeds and are known to provide health benefits to the host. These live microbes manipulate the gut microbes by suppressing the growth and competing with other intestinal microbes. Lactic acid bacteria (LAB) are potential probiotics that have been widely studied; in this study, we have elucidated the effects of two LAB types (LAB1 & LAB2) isolated from the intestinal content of farmed healthy juveniles of rainbow trout on the distal intestinal microbial communities of Atlantic salmon (Salmo salar). We employed high-throughput 16S rRNA gene amplicon sequencing technique to investigate the intestinal bacterial communities harbouring in the distal intestinal content and mucus of the 3 groups of Atlantic salmon fed diets-devoid of or diets-containing LABs. Our results show that LAB shifts the intestinal microbial profile; LAB supplementation did not show any significant alterations in the alpha diversity of the three groups. However, LAB2 feeding increased the bacterial diversity in the distal intestinal mucus of the fish. Beta diversity analysis showed a significant difference between control and LAB-fed groups. We found intestinal Lactobacillus abundant and dominant in the LAB-fed fish. In addition, few members of the phyla Tenericutes, Actinobacteria, and Spirochaetes were also found to be abundant in the LAB-fed groups. Furthermore, the bacterial association network analysis showed that in the LAB1-fed group significantly abundant and relevant OTUs are closely linked suggesting that they may be highly interactive. We did not find any evidence on cooperation-related runaway effect. These results indicate that dietary probiotics modulate the distal intestinal microbiota of Atlantic salmon. These results are intended to improve the understanding of the modulations and interactions of gut microbiota under the influence of selected probiotic species.",
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