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"study-abstract": "We examined the bacterial flora associated with 20 cultured strains of Ectocarpus sp. from different locations, and recorded both the bacterial diversity associated directly to the alga and that found in the culture medium. Experiments were carried out to amplify an approximately 400 bp fragment of the bacterial 16S comprising the V3 and V4 regions and sequenced on the Illumina MiSeq platform using V2 reagents ( 2x250 bp reads).",
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"study-abstract": "We aimed to explore the community composition and turnover of eukaryotic and bacterial\n microorganisms associated with ice-associated and sinking algal aggregates, as well as their similarity\n to potential source communities of sea ice, water and deep-sea sediments using Illumina tag sequencing.\n We sampled algae aggregates growing in melt ponds on sea ice, deposited algae aggregates at the seafloor\n in more than 4000 m water depth, sea ice, upper water column, sediment surface and the gut content of\n holothurians feeding on the deposits.",
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"study-abstract": "Coccolithophores are photosynthetic marine microorganisms that cover their eukaryotic cells with calcium carbonate disks. They are of great importance in earth's biogeochemical cycles of oxygen, carbon and sulfur and are essential in marine primary production. The species Emiliania huxleyi is the most abundant coccolithophore micro-alga in modern-day oceans. It creates vast annual blooms that stretch for thousands of square kilometers and can be viewed by satellites from space. These dense blooms end after several weeks with the collapse of the algal population. The collapse of the bloom is usually attributed to viral infections or environmental changes. Recent studies demonstrated that bacteria play an important role in the life and death of E. huxleyi populations. In controlled laboratory experiments, it was shown that the bacterium Phaeobacter inhibens has a dynamic mutualistic-pathogenic interaction with the alga. In a co-culture model system, the microbes first exchange beneficial metabolites, however once the alga ages, the bacterium kills its algal partner. This interaction might contribute to the known E. huxleyi bloom dynamics. Nevertheless, in order to interpret data acquired in the lab, a combination between laboratory findings and field studies will be most beneficial in bridging the complex natural environment of E. huxleyi with the laboratory model system. In this current research I intend to link between lab observations and the marine environment. In order to do so, the identity and relative abundance of bacteria that naturally associate with a stable E. huxleyi population in the gulf of Aqaba will be assessed seasonally in order to account for possible seasonal changes in bacterial composition. In understating who are the bacterial key players in the natural environment of E. huxleyi I hope to attain a wide and unbiased comprehensive view of natural algal-bacterial interactions.",
"study-name": "Natural Bacterial Communities Associated with Emiliania huxleyi",
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"study-abstract": "The mixed culture metagenome Third Party Annotation (TPA) assembly was derived from the primary whole genome shotgun (WGS) data set: PRJNA406753. This project includes samples from the following biomes: Host-associated, Algae.",
"study-name": "EMG produced TPA metagenomics assembly of the Chrysochromulina tobin associated microbial communities from unialgal haptophyte culture, Seattle, Washington, USA - P5_32mM MetaG metagenome (mixed culture metagenome) data set.",
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"last-update": "2019-06-12T20:03:33",
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"study-abstract": "The mixed culture metagenome Third Party Annotation (TPA) assembly was derived from the primary whole genome shotgun (WGS) data set: PRJNA406751. This project includes samples from the following biomes: Host-associated, Algae.",
"study-name": "EMG produced TPA metagenomics assembly of the Chrysochromulina tobin associated microbial communities from unialgal haptophyte culture, Seattle, Washington, USA - P5_8mM MetaG metagenome (mixed culture metagenome) data set.",
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"secondary-accession": "ERP021699",
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"study-abstract": "Over the last decade, disease has emerged from being a long overlooked factor in natural communities to become an increasing focus for ecological research in terrestrial and marine ecosystems. There is also major concern that global warming and other anthropogenic stressors may be responsible for the spread of pathogens, enhanced virulence and decreased resilience of hosts. This project focuses on macroalgae or seaweeds, the dominant habitat-forming organisms in temperate rocky reef communities worldwide. They are the trees of temperate coastal ecosystems. Anthropogenic stressors can have dramatic effects on the health and persistence of large macroalgae and are responsible for extensive losses of habitat-forming algae. Importantly, declines in habitat-forming macroalgae not only impact the algae themselves, but have major effects on the diversity and abundance of associated organisms. During this project, we aim to characterise microbial communities associated with the surfaces of dominant seaweed species on temperate Australian coasts, from northern New South Wales, south to Tasmania and also along the western Australian coastline. Furthermore, we aim to identify potential pathogens associated with stressed specimens and quantify their abundance at different sites and different times and correlate microbial data with various physico-chemical information from each site and specimen. This work will provide baseline data on algal-bacterial associations on a latitudinal scale and may also provide information on the distribution and abundance of potential algal pathogens. We hope also to understand how different environmental conditions influence algal-associated microorganisms and predict how environmental change may affect interactions between these microbes and their hosts.",
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"study-name": "EMG produced TPA metagenomics assembly of the Marine algal microbial communities from Sidmouth, United Kingdom - Sidmouth_Asex2 metaG metagenome (marine metagenome) data set.",
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"secondary-accession": "ERP108248",
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"study-abstract": "The marine metagenome Third Party Annotation (TPA) assembly was derived from the primary whole genome shotgun (WGS) data set: PRJNA365151. This project includes samples from the following biomes: Host-associated, Algae, Red algae.",
"study-name": "EMG produced TPA metagenomics assembly of the Marine algal microbial communities from Porto, Italy - Porto_4 metaG metagenome (marine metagenome) data set.",
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"public-release-date": null,
"study-abstract": "Restructuring of epibacterial communities on Fucus vesiculosus forma mytili in response to elevated pCO2 and increased temperature levels",
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"study-abstract": "Bacteria attached to a diatom host cell are thought to operate as potential symbionts, functioning as mutualistic, parasitic, or commensal epibionts. This study investigates if similar relationships can be found at the level of the individual diatom host cell by analyzing the metagenomes of six representative Thalassiosira host cells with dissimilar associated bacteria. Diatoms were collected from the deep chlorophyll maximum in the oligotrophic waters of Station ALOHA (22° 45\"N, 158° 00\"W), isolated using fluorescently activated cell sorting, and DNA amplified using multiple displacement amplification. Preliminary analysis of the 16S rDNA of 32 host cells found that the composition of bacterial associations could be divided into three distinct groups; six cells from the two most common groupings were selected for further analysis. This initial metagenomic analysis explores nutrient pathways, antibiotic production, quorum sensing, motility, and other genes that may be essential to the diatom-bacterial symbiotic interaction. This work surveys the potential genetic contribution of bacteria, diatoms, as well as community-level interactions that determine the health of the diatom host cell and their bacterial consortia.",
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