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"study-abstract": "Here we examined the microbial communities in soils from both the organic and mineral layer from 18 sites in six ecozones across North America. This is 16S rRNA gene data",
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"study-abstract": "Decomposition and storage of plant-derived carbon has become a hot topic in environmental research. Since carbon dioxide has been detected as one of the most relevant drivers of global climate change, its potential storage and fate in soils has become key for predicting climate scenarios. Unfortunately, studies with a focus on the decomposition and driving factors are still lacking for precise modelling of the fluxes in a changing world. The main actors of carbon degradation in soils have not been fully identified, especially in regard to bacteria very little is known about their contribution to degradation of persistent carbon components. In the present study, the active microbial community involved in degrading 13C-labelled cellulose and lignin in forest soils was identified using a DNA stable isotopic probing (SIP) approach. Microbial activity assessed by measurements of 13CO2 increased with labelled substrate addition, during a 28 days incubation period. Within the vast microbial communities, bacterial and fungal members specialized in degrading cellulose (e.g. Devosia (Rhizobiales), Sebacinales), lignin (e.g. Sphingopyxis (Sphingomonadales), Paucibacter (Burkholderiales), Xylariales, Auriculariales, Helotiales), and both substrates (e.g. Caulobacter (Caulobacterales), Schizoporaceae, Orbiliales) have been identified. Over the course of the experiment, a successional shift from early degrading colonizers (e.g. Brevundimonas (Caulobacterales), Trichosporonales, Hymenochaetales, Gomphales) to late stage beneficiary microorganisms (e.g. Sphingopyxis (Sphingomonadales), Devosia (Rhizobiales), Orbiliales) became evident. These results provide novel insights into the degradation processes of woody plant debris in soil, disentangling the active microbial degraders from the beneficiaries, within the vast complexity of the soil microbiome.",
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"study-abstract": "The purpose of this study was to explore the diversity of ammonia-oxidizing microbes (bacteria and archaea) in the soils at Coweeta LTER, a research site in western North Carolina.",
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"secondary-accession": "SRP045746",
"centre-name": "The University of Findlay",
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"study-abstract": "The flow of energy within an ecosystem can be considered either top-down, where predators influence consumers, or bottom-up, where producers influence consumers. Plethodon cinereus (Red-backed Salamander) is a terrestrial keystone predator who feeds on invertebrates within the ecosystem. We investigated the impact of the removal of P. cinereus on the detritivore food web in an upland deciduous forest in northwest Ohio, USA. A total of eight aluminum enclosures, each containing a single P. cinereus under a small log, were constructed in the deciduous forest. On Day 1 of the experiment, four salamanders were evicted from four of the eight enclosures. Organic matter and soil were collected from the center of each enclosure at Day 1 and Day 21. From each sample, DNA was extracted, fungal-specific amplification performed, and 454 pyrosequencing was used to sequence the nuclear ribosomal internal transcribed spacer (ITS2) region and partial ribosomal large subunit (LSU). Changes in overall fungal community composition or species diversity were not statistically significant between treatments. Statistically significant shifts in the most abundant taxonomic groups of fungi were documented in presence but not absence enclosures. We concluded that P. cinereus does not affect the overall composition or diversity of fungal communities, but does have an impact on specific groups of fungi. This study used a metagenomics-based approach to investigate a missing link among a keystone predator, P. cinereus, invertebrates, and fungal communities, all of which are critical in the detritivore food web.",
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"study-abstract": "Soil samples have been collected in a truffle orchard located in the Vosges (France). DNA was extracted and used to decipher the structure of the bacterial communities along truffle maturation.",
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"study-abstract": "It is well known that an intensive conventional farming and continuous use of land resources can lead to a decrease of soil sustainability. The aim of this study was to compare the impact of four different tillage practices (minimum tillage MT, shallow ploughing SP, ripper subsoiling RS and conventional tillage DP) on soil profile under both continuous and rotating (wheat, bean, maize) maize production since 1970. The field study was conducted on 24 experimental plots on a loam soil of Fagna, Tuscany (Italy), where soil samples were collected at four different layers of 10 cm each, up to a total depth of 40 cm (0-10, 10-20, 20-30 and 30-40 cm in triplicate) and of 20 cm (0-10 and 10-20 cm) from continuous and rotating maize fields respectively, in order to measure the effect of the different tillage practices on soil organic matter, microbial diversity and microbial activity.",
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