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InterPro-Version: 108.0
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{
"metadata": {
"accession": "P9WEG5",
"id": "MFMF_ANNMO",
"source_organism": {
"taxId": "326622",
"scientificName": "Annulohypoxylon moriforme",
"fullName": "Annulohypoxylon moriforme (Filamentous fungus)"
},
"name": "Cytochrome P450 monooxygenase mfmF",
"description": [
"Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of the phthalide-terpenoid hybrid 11'-O-desmethylfendlerol (PubMed:38404388). Within the pathway, mfmF catalyzes C-3 hydroxylation of 5-hydroxy-4-(hydroxymethyl)-7-methoxy-6-methylphthalide to yield cyclopolic acid (PubMed:38404388). The biosynthesis of 11'-O-desmethylfendlerol begins with the NR-PKS mfmB that forms 3,5-dimethylorsellinic acid (DMOA), which is then transformed into the phthalide 5,7-dihydroxy-4-(hydroxymethyl)-6-methylphthalide by the cytochrome P450 monooxygenase mfmA and the hydrolase mfmC. Subsequently, the methyltransferase mfmE catalyzes 7-O-methylation to yield 5-hydroxy-4-(hydroxymethyl)-7-methoxy-6-methylphthalide, which undergoes C-3 hydroxylation by the cytochrome P450 monooxygenase mfmF. The resultant cyclopolic acid (2,5-dihydroxy-4-(hydroxymethyl)-7-methoxy-6-methylphthalide) is then farnesylated by the DMATS-type prenyltransferase mfmD to afford 5-O-farnesylcyclopolic acid. Finally, the Pyr4-family terpene cyclase mfmH cyclizes the farnesyl moiety of 5-O-farnesylcyclopolic acid into a drimane-like structure, thus completing the biosynthesis of 11'-O-desmethylfendlerol (PubMed:38404388)"
],
"length": 493,
"sequence": "MWSLIPLTAIVLVLVAILHRLFFHPLSHVPGPFLARFSSLYLHSICYLGIEATVLRQLHIKYKTKVLRVAPNSVSISDSEAVGEIYVRGGGFPKDDRYKNFNLGPIVSIFSSLDREYRDVRAKAVAPLFSPAQLRAESGPQGVIGGCIAEFVDQLRKSKEAGIGADLMDMCARLSIDVVTGYLLGQKYGGLQENAHLPPQERQSHKLSANPFIFSIVAFSRFSLLPHRIFQFLYSTSQRLSTNDEVTESFLKLDRFTNNVLEGTTAGEKVSEKPNGGYYHERLISQAGISRMEAAAQSQAVLFAGADSTAVMLATILFHLVQNGAARSRLLCEVRSDKPQDMRFLRAVVKEGLRLGMANPTRMTRVVPKSGPGLRVGDVLLSPGTIVGCAAYNLHHDPEVFPDPFTFRPERWLDDGTDRGLRRPGMEKSMIPFGVGSRACIGKNLAQQQLHDTVVAVIDSEVLEGARTCQERIEIIEWFNADIKGHHLDIKWS",
"proteome": null,
"gene": "mfmF",
"go_terms": [
{
"identifier": "GO:0004497",
"name": "monooxygenase activity",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0005506",
"name": "iron ion binding",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0016705",
"name": "oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0020037",
"name": "heme binding",
"category": {
"code": "F",
"name": "molecular_function"
}
}
],
"protein_evidence": 1,
"source_database": "reviewed",
"is_fragment": false,
"in_alphafold": true,
"in_bfvd": false,
"ida_accession": "a02e61ba6c64bb9a9c779746e03a6c43264a16fb",
"counters": {
"domain_architectures": 520850,
"entries": 13,
"isoforms": 0,
"proteomes": 0,
"sets": 1,
"structures": 0,
"taxa": 1,
"dbEntries": {
"cathgene3d": 1,
"ssf": 1,
"cdd": 1,
"pfam": 1,
"panther": 1,
"prints": 2,
"prosite": 1,
"interpro": 5
},
"proteome": 0,
"taxonomy": 1,
"similar_proteins": 520850
}
}
}
