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{
"metadata": {
"accession": "P00959",
"id": "SYM_ECOLI",
"source_organism": {
"taxId": "83333",
"scientificName": "Escherichia coli (strain K12)",
"fullName": "Escherichia coli (strain K12)"
},
"name": "Methionine--tRNA ligase",
"description": [
"Catalyzes the attachment of L-methionine to tRNA(Met) (PubMed:216545, PubMed:367445, PubMed:4297674, PubMed:4581817, PubMed:7932711). Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (PubMed:216545, PubMed:4581817). MetRS can indeed attach methionine to tRNA(mMet), which supplies methionine for elongation of nascent peptides, and to tRNA(fMet), which supplies formylmethionine for initiation of peptide synthesis (PubMed:216545, PubMed:4581817). However, MetRS charges tRNA(fMet) with a greater efficiency than tRNA(mMet) in several strains of E.coli, suggesting a preference for initiation of new peptides (PubMed:216545). Cannot use D-methionine (PubMed:4297674)",
"In addition, MetRS can reject misactivated homocysteine by a tRNA-independent hydrolysis of the homocysteinyl-AMP intermediate, preventing incorporation of homocysteine into tRNA and proteins (PubMed:2191291, PubMed:7024910). The homocysteinyl-AMP intermediate undergoes an intramolecular cyclization reaction in which the thiolate side chain of homocysteine displaces the AMP group, forming homocysteine-thiolactone and AMP (PubMed:2191291, PubMed:7024910)"
],
"length": 677,
"sequence": "MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGHEVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEENRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCEVCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANKMQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSVSFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPSNLFVHGYVTVNGAKMSKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALADLANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLNTELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDPIQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK",
"proteome": "UP000000625",
"gene": "metG",
"go_terms": [
{
"identifier": "GO:0004825",
"name": "methionine-tRNA ligase activity",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0006431",
"name": "methionyl-tRNA aminoacylation",
"category": {
"code": "P",
"name": "biological_process"
}
},
{
"identifier": "GO:0000166",
"name": "nucleotide binding",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0004812",
"name": "aminoacyl-tRNA ligase activity",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0005524",
"name": "ATP binding",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0006418",
"name": "tRNA aminoacylation for protein translation",
"category": {
"code": "P",
"name": "biological_process"
}
},
{
"identifier": "GO:0000049",
"name": "tRNA binding",
"category": {
"code": "F",
"name": "molecular_function"
}
}
],
"protein_evidence": 1,
"source_database": "reviewed",
"is_fragment": false,
"in_alphafold": true,
"in_bfvd": false,
"ida_accession": "605e59fa9284a45ce9616dfe8a2213f70331198f",
"counters": {
"domain_architectures": 13265,
"entries": 34,
"isoforms": 0,
"proteomes": 1,
"sets": 6,
"structures": 24,
"taxa": 1,
"dbEntries": {
"ssf": 4,
"cdd": 3,
"cathgene3d": 4,
"pfam": 3,
"profile": 1,
"panther": 1,
"ncbifam": 3,
"hamap": 1,
"prosite": 1,
"prints": 1,
"interpro": 12
},
"proteome": 1,
"taxonomy": 1,
"similar_proteins": 13265
}
}
}
