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{
"metadata": {
"accession": "A0A2Z4HPY5",
"id": "EFUC_HORCR",
"source_organism": {
"taxId": "284138",
"scientificName": "Hormonema carpetanum",
"fullName": "Hormonema carpetanum"
},
"name": "Acetyltransferase efuC",
"description": [
"Acetyltransferase; part of the gene cluster that mediates the biosynthesis of enfumafungin, a glycosylated fernene-type triterpenoid with potent antifungal activity, mediated by its interaction with beta-1,3-glucan synthase and the fungal cell wall (PubMed:30051576). The pathway begins with the terpene cyclase-glycosyl transferase fusion protein that most likely uses 2,3-oxidosqualene as substrate and catalyzes glycosylation immediately after cyclization (Probable). The fernene glycoside then could be processed by the desaturase efuI which catalyzes isomerization of a double bond established by efuA to form the core structure (Probable). The latter would then undergo a series of hydroxylations in unknown order at C-2, C-19, C-23 and C-25, which would be catalyzed by two of the three cytochrome P450 monooxygenases efuB, efuG or efuH (Probable). The hydroxy-group at C-25 becomes oxidized by the dehydrogenase efuE to enable a spontaneous, non-enzymatic hemiacetal formation with C-23 (Probable). After hydroxylation at C-2, acetylation by the acetyltransferase efuC takes place (Probable). The final steps in enfumafungin biosynthesis require expansion of the 5-membered ring by lactonization via a Baeyer-Villiger reaction mediated by one of the BGC's cytochrome P450 monooxygenases (efuB, efuG or efuH) followed by ring cleavage (Probable). This type of reaction would establish a double bond between C-20 and C-21 which could be reduced by the reductase efuL to form the final product (Probable)"
],
"length": 551,
"sequence": "MSAPRTALSSARQLSNGLIRRTNPRKRFLPLPSTVRSFTNNKPLRSQATAAQEPIVESERPSPVTSVNQPLSFPCLDALDTKTQSLTKQKKSLSGPEPSYAAGETEHFHSASPILLDWGGVLPEFDIAYETWGTLNADKSNAILLHTGLSASSHAKSTAKNPKPGWWEKFIGPGAPLDTDKYHIICTNVLGGCYGSTGPSSIDPLAENNTSSAQKDRYATRFPILTMQDMVRAQFRLLDWMGIDALYASVGSSMGGMQSLAACVLEPSRVGKVVSVSGCARSHPYSIAMRHTQRQVLMNDPAWSSTRGNYYPSIPPHAGMKLAREIATVTYRSGPEWEQRFGRRRADPSKPPALCPDFLIETYLDHAGEKWCLEYDANSLLYVSKAMDLFDLGDEHMRTLDGVRKSNAWKLDGFAAGTAKEEEKAGLCNLTLPDTPYEEQDETSSELLASSPEEAKRARETSSAALIAEEPPEDLVKGLQALSGTPALVMGVASDILFPAWQQREIAAALRKAGNRRVTHVELGEDRSLFGHDTFLLDLEGVGGEIRRFLG",
"proteome": null,
"gene": "efuC",
"go_terms": [
{
"identifier": "GO:0016747",
"name": "acyltransferase activity, transferring groups other than amino-acyl groups",
"category": {
"code": "F",
"name": "molecular_function"
}
},
{
"identifier": "GO:0009058",
"name": "biosynthetic process",
"category": {
"code": "P",
"name": "biological_process"
}
}
],
"protein_evidence": 3,
"source_database": "reviewed",
"is_fragment": false,
"in_alphafold": true,
"in_bfvd": false,
"ida_accession": "4208e7859dea7ff67287230d6f84ba11d78c698d",
"counters": {
"domain_architectures": 386493,
"entries": 10,
"isoforms": 0,
"proteomes": 0,
"sets": 1,
"structures": 0,
"taxa": 1,
"dbEntries": {
"cathgene3d": 1,
"ssf": 1,
"pfam": 1,
"panther": 1,
"pirsf": 1,
"hamap": 1,
"ncbifam": 1,
"interpro": 3
},
"proteome": 0,
"taxonomy": 1,
"similar_proteins": 386493
}
}
}
