{"metadata":{"accession":"A0A2Z4HPY5","id":"EFUC_HORCR","source_organism":{"taxId":"284138","scientificName":"Hormonema carpetanum","fullName":"Hormonema carpetanum"},"name":"Acetyltransferase efuC","description":["Acetyltransferase; part of the gene cluster that mediates the biosynthesis of enfumafungin, a glycosylated fernene-type triterpenoid with potent antifungal activity, mediated by its interaction with beta-1,3-glucan synthase and the fungal cell wall (PubMed:30051576). The pathway begins with the terpene cyclase-glycosyl transferase fusion protein that most likely uses 2,3-oxidosqualene as substrate and catalyzes glycosylation immediately after cyclization (Probable). The fernene glycoside then could be processed by the desaturase efuI which catalyzes isomerization of a double bond established by efuA to form the core structure (Probable). The latter would then undergo a series of hydroxylations in unknown order at C-2, C-19, C-23 and C-25, which would be catalyzed by two of the three cytochrome P450 monooxygenases efuB, efuG or efuH (Probable). The hydroxy-group at C-25 becomes oxidized by the dehydrogenase efuE to enable a spontaneous, non-enzymatic hemiacetal formation with C-23 (Probable). After hydroxylation at C-2, acetylation by the acetyltransferase efuC takes place (Probable). The final steps in enfumafungin biosynthesis require expansion of the 5-membered ring by lactonization via a Baeyer-Villiger reaction mediated by one of the BGC's cytochrome P450 monooxygenases (efuB, efuG or efuH) followed by ring cleavage (Probable). This type of reaction would establish a double bond between C-20 and C-21 which could be reduced by the reductase efuL to form the final product (Probable)"],"length":551,"sequence":"MSAPRTALSSARQLSNGLIRRTNPRKRFLPLPSTVRSFTNNKPLRSQATAAQEPIVESERPSPVTSVNQPLSFPCLDALDTKTQSLTKQKKSLSGPEPSYAAGETEHFHSASPILLDWGGVLPEFDIAYETWGTLNADKSNAILLHTGLSASSHAKSTAKNPKPGWWEKFIGPGAPLDTDKYHIICTNVLGGCYGSTGPSSIDPLAENNTSSAQKDRYATRFPILTMQDMVRAQFRLLDWMGIDALYASVGSSMGGMQSLAACVLEPSRVGKVVSVSGCARSHPYSIAMRHTQRQVLMNDPAWSSTRGNYYPSIPPHAGMKLAREIATVTYRSGPEWEQRFGRRRADPSKPPALCPDFLIETYLDHAGEKWCLEYDANSLLYVSKAMDLFDLGDEHMRTLDGVRKSNAWKLDGFAAGTAKEEEKAGLCNLTLPDTPYEEQDETSSELLASSPEEAKRARETSSAALIAEEPPEDLVKGLQALSGTPALVMGVASDILFPAWQQREIAAALRKAGNRRVTHVELGEDRSLFGHDTFLLDLEGVGGEIRRFLG","proteome":null,"gene":"efuC","go_terms":[{"identifier":"GO:0016747","name":"acyltransferase activity, transferring groups other than amino-acyl groups","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0009058","name":"biosynthetic process","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"4208e7859dea7ff67287230d6f84ba11d78c698d","counters":{"domain_architectures":386493,"entries":10,"isoforms":0,"proteomes":0,"sets":1,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":1,"ssf":1,"pfam":1,"panther":1,"pirsf":1,"hamap":1,"ncbifam":1,"interpro":3},"proteome":0,"taxonomy":1,"similar_proteins":386493}}}