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{
"metadata": {
"accession": "IPR038633",
"entry_id": null,
"type": "homologous_superfamily",
"go_terms": null,
"source_database": "interpro",
"member_databases": {
"cathgene3d": {
"G3DSA:2.30.29.70": "Proteasomal ubiquitin receptor Rpn13/ADRM1"
}
},
"integrated": null,
"hierarchy": {
"accession": "IPR038633",
"name": "Proteasomal ubiquitin receptor Rpn13/ADRM1, Pru domain superfamily",
"type": "Homologous_superfamily",
"children": []
},
"name": {
"name": "Proteasomal ubiquitin receptor Rpn13/ADRM1, Pru domain superfamily",
"short": "Rpn13/ADRM1_Pru_sf"
},
"description": [
{
"text": "<p>This superfamily includes Rpn13 from budding yeasts and its homologue, ADRM1 from animals.</p>\n\n<p>Rpn13 is a subunit and an ubiquitin receptor of the 19S regulatory particle of the 26S proteasome lid. The 26S proteasome is a huge macromolecular protein-degradation machine consisting of a proteolytically active 20S core, in the form of four disc-like proteins, and one or two 19S regulatory particles. The regulatory particle(s) sit on the top and or bottom of the core, de-ubiquitinate the substrate peptides, unfold them and guide them into the narrow channel through the centre of the core. Rpn13 and its homologues dock onto the regulatory particle through the N-terminal region which binds Rpn2. The C-terminal part of the domain binds de-ubiquitinating enzyme Uch37/UCHL5 and enhances its isopeptidase activity. Rpn13 binds ubiquitin via a conserved amino-terminal region called the pleckstrin-like receptor for ubiquitin, termed Pru, domain [[cite:PUB00053442]]. The domain forms two contiguous anti-parallel β-sheets with a configuration similar to the pleckstrin-homology domain (PHD) fold [[cite:PUB00049233], [cite:PUB00054318]]. Rpn13's ability to bind ubiquitin and the proteasome subunit Rpn2/S1 simultaneously supports evidence of its role as a ubiquitin receptor. Finally, when complexed to di-ubiquitin, via the Pru, and Uch37 via the C-terminal part, it frees up the distal ubiquitin for de-ubiquitination by the Uch37 [[cite:PUB00049233]].</p>",
"llm": false,
"checked": false,
"updated": false
},
{
"text": "<p>This superfamily represents the Pru (pleckstrin-like receptor for the Ub) domain.</p>",
"llm": false,
"checked": false,
"updated": false
}
],
"wikipedia": null,
"literature": {
"PUB00054318": {
"PMID": 20471946,
"ISBN": null,
"volume": "38",
"issue": "3",
"year": 2010,
"title": "Structure of proteasome ubiquitin receptor hRpn13 and its activation by the scaffolding protein hRpn2.",
"URL": null,
"raw_pages": "404-15",
"medline_journal": "Mol Cell",
"ISO_journal": "Mol. Cell",
"authors": [
"Chen X",
"Lee BH",
"Finley D",
"Walters KJ."
],
"DOI_URL": "http://dx.doi.org/10.1016/j.molcel.2010.04.019"
},
"PUB00053442": {
"PMID": 18497817,
"ISBN": null,
"volume": "453",
"issue": "7194",
"year": 2008,
"title": "Proteasome subunit Rpn13 is a novel ubiquitin receptor.",
"URL": null,
"raw_pages": "481-8",
"medline_journal": "Nature",
"ISO_journal": "Nature",
"authors": [
"Husnjak K",
"Elsasser S",
"Zhang N",
"Chen X",
"Randles L",
"Shi Y",
"Hofmann K",
"Walters KJ",
"Finley D",
"Dikic I."
],
"DOI_URL": "http://dx.doi.org/10.1038/nature06926"
},
"PUB00049233": {
"PMID": 18497827,
"ISBN": null,
"volume": "453",
"issue": "7194",
"year": 2008,
"title": "Ubiquitin docking at the proteasome through a novel pleckstrin-homology domain interaction.",
"URL": null,
"raw_pages": "548-52",
"medline_journal": "Nature",
"ISO_journal": "Nature",
"authors": [
"Schreiner P",
"Chen X",
"Husnjak K",
"Randles L",
"Zhang N",
"Elsasser S",
"Finley D",
"Dikic I",
"Walters KJ",
"Groll M."
],
"DOI_URL": "http://dx.doi.org/10.1038/nature06924"
}
},
"set_info": null,
"overlaps_with": [
{
"accession": "IPR044868",
"name": "Rpn13/ADRM1, Pru domain",
"type": "domain"
},
{
"accession": "IPR006773",
"name": "Proteasomal ubiquitin receptor Rpn13/ADRM1",
"type": "family"
}
],
"counters": {
"subfamilies": 0,
"domain_architectures": 0,
"interactions": 0,
"matches": 6083,
"pathways": 393,
"proteins": 6062,
"proteomes": 3139,
"sets": 0,
"structural_models": {
"alphafold": 5567,
"bfvd": 0
},
"structures": 42,
"taxa": 10876
},
"entry_annotations": {},
"cross_references": {},
"is_llm": false,
"is_reviewed_llm": false,
"is_updated_llm": false,
"representative_structure": {
"accession": "5v1y",
"name": "Crystal structure of the ternary RPN13 PRU-RPN2 (940-953)-ubiquitin complex"
}
}
}
