An RNAi-based high content screening microscopy platform was used to screen a genome-wide library of over 51,000 small interfering RNAs (siRNAs) targeting approximately 22,000 human genes for interference with ER-to-plasma membrane transport of the well-characterized secretory cargo membrane protein tsO45G. This approach allows single-cell quantification of the relative levels of fluorescently labelled tsO45G both inside the cell and at the plasma membrane
