IHECRE00000044.3 PREVIOUS JSON  

Species Homo sapiens
Type Single donor
Status Partial
Project BLUEPRINT
Description macrophage - T=6days B-glucan from venous blood of donor: N00031319896021
Live version IHECRE00000044.4
Previous versions IHECRE00000044.2   IHECRE00000044.1  

Metadata


Biomaterial type Primary Cell Culture
Donor id N00031319896021
Donor ethnicity Northern European
Donor sex Female
Gender female
Tissue type venous blood
Taxon id 9606
Disease None
Sample ontology uri http://purl.obolibrary.org/obo/CL_0000235
Donor age 30 - 35
Treatment T=6days B-glucan
Culture conditions PBS supplemented with 1% PBA and antibodies: anti-CD45-PeCy7 (Beckman Coulter), anti-CD14-FITC (Beckman Coulter), anti-CD3-PC5 (Biolegend (ITK)), anti-CD19-ECD (Beckman Coulter) and anti-56-PE (BD Bioscience)
Cell type macrophage
Donor health status Healthy
Biomaterial provider Sanquin Blood bank, Nijmegen, the Netherlands

Experimental data


Assay Type Experiment Type Archive Primary ID Secondary ID URL
ChIP-Seq Histone H3K4me3 EGA EGAX00001215521 EGAD00001002314 https://ega-archive.org/datasets/EGAD00001002314
ChIP-Seq Histone H3K27ac EGA EGAX00001215523 EGAD00001002314 https://ega-archive.org/datasets/EGAD00001002314
DNase-Hypersensitivity Chromatin Accessibility EGA EGAX00001215904 EGAD00001002425 https://ega-archive.org/datasets/EGAD00001002425
RNA-Seq total-RNA-Seq EGA EGAX00001216493 EGAD00001002522 https://ega-archive.org/datasets/EGAD00001002522
ChIP-Seq Histone H3K4me1 EGA EGAX00001215522 EGAD00001002314 https://ega-archive.org/datasets/EGAD00001002314