IHECRE00000044.2 PREVIOUS JSON  

Species Homo sapiens
Type Single donor
Status Partial
Project BLUEPRINT
Description macrophage - T=6days B-glucan from Venous blood of N00031319896021
Live version IHECRE00000044.4
Previous versions IHECRE00000044.1  

Metadata


Biomaterial type Primary cell culture
Donor id N00031319896021
Donor ethnicity Northern European
Donor sex Female
Tissue type Venous blood
Taxon id 9606
Sample ontology uri http://purl.obolibrary.org/obo/CL_0000235
Disease None
Donor age 30 - 35
Treatment T=6days B-glucan
Culture conditions PBS supplemented with 1% PBA and antibodies: anti-CD45-PeCy7 (Beckman Coulter), anti-CD14-FITC (Beckman Coulter), anti-CD3-PC5 (Biolegend (ITK)), anti-CD19-ECD (Beckman Coulter) and anti-56-PE (BD Bioscience)
Cell type macrophage
Markers CD3-,CD19-,CD56-
Biomaterial provider Sanquin Blood bank, Nijmegen, the Netherlands
Donor health status Healthy

Experimental data


Assay Type Experiment Type Archive Primary ID Secondary ID URL
RNA-Seq mRNA-seq EGA EGAX00001216493 EGAD00001001582 https://ega-archive.org/datasets/EGAD00001001582
DNase-Hypersensitivity Chromatin Accessibility EGA EGAX00001215904 EGAD00001001581 https://ega-archive.org/datasets/EGAD00001001581