PDBsum entry 5fmf

Transcription

PDB id: 5fmf

Contents

Protein chains

473 a.a.

174 a.a.

1422 a.a.

1156 a.a.

266 a.a.

178 a.a.

214 a.a.

84 a.a.

171 a.a.

133 a.a.

119 a.a.

65 a.a.

115 a.a.

46 a.a.

116 a.a.

101 a.a.

293 a.a.

180 a.a.

118 a.a.

121 a.a.

150 a.a.

174 a.a.

62 a.a.

63 a.a.

578 a.a.

DNA/RNA

Metals

_ZN ×9

_MG ×2

References listed in PDB file

Key reference

• Title: Structure of an RNA polymerase ii preinitiation complex.
• Authors: K.Murakami, K.L.Tsai, N.Kalisman, D.A.Bushnell, F.J.Asturias, R.D.Kornberg.
• Ref.: Proc Natl Acad Sci U S A, 2015, 112, 13543-13548. [DOI no: 10.1073/pnas.1518255112]
• PubMed id: 26483468

Abstract

The structure of a 33-protein, 1.5-MDa RNA polymerase II preinitiation complex (PIC) was determined by cryo-EM and image processing at a resolution of 6-11 Å. Atomic structures of over 50% of the mass were fitted into the electron density map in a manner consistent with protein-protein cross-links previously identified by mass spectrometry. The resulting model of the PIC confirmed the main conclusions from previous cryo-EM at lower resolution, including the association of promoter DNA only with general transcription factors and not with the polymerase. Electron density due to DNA was identifiable by the grooves of the double helix and exhibited sharp bends at points downstream of the TATA box, with an important consequence: The DNA at the downstream end coincides with the DNA in a transcribing polymerase. The structure of the PIC is therefore conducive to promoter melting, start-site scanning, and the initiation of transcription.