PDBsum entry 5yo1

Hydrolase

PDB id: 5yo1

Contents

Protein chain

866 a.a.

Ligands

PUY ×2

GOL ×12

Metals

_ZN

_NA

Waters ×987

PDB id:

5yo1

Name:

Hydrolase

Title:

Structure of epepn e298a mutant in complex with puromycin

Structure:

Aminopeptidase n. Chain: a. Synonym: alpha-aminoacylpeptide hydrolase, e. Coli amino peptidase n. Engineered: yes. Mutation: yes

Source:

Escherichia coli k12. Organism_taxid: 83333. Strain: k12. Gene: pepn, b0932, jw0915. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.50Å R-factor: 0.129 R-free: 0.184

Authors:

R.J.Ganji,R.Reddi,A.K.Marapaka,A.Addlagatta

Key ref:

R.J.Ganji et al. Molecular determinants of selective inhibition of hum puromycin sensitive aminopeptidase by puromycin. To be published, .

Date:

26-Oct-17 Release date: 07-Nov-18

Protein chain

P04825  (AMPN_ECOLI) -  Aminopeptidase N from Escherichia coli (strain K12)

Seq: 870 a.a.

Struc: 866 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.11.2 - membrane alanyl aminopeptidase.
• Reaction: Release of an N-terminal amino acid, Xaa-|-Xbb- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
• Cofactor: Zn(2+)