PDBsum entry 5arc

Hydrolase

PDB id: 5arc

Contents

Protein chain

269 a.a.

Ligands

EI3

SO4

GOL

Metals

_CA

Waters ×319

PDB id:

5arc

Name:

Hydrolase

Title:

Cooperative bio-metallic selectivity in a tailored protease enables creation of a c-c cross-coupling heckase

Structure:

Subtilisin savinase. Chain: a. Fragment: mature peptide chain, residues 1-269. Synonym: alkaline protease, subtilisin. Engineered: yes. Mutation: yes

Source:

Bacillus lentus. Organism_taxid: 1467. Expressed in: bacillus subtilis. Expression_system_taxid: 1423

Resolution:

1.10Å R-factor: 0.109 R-free: 0.123

Authors:

M.Sharma,A.Diaz-Rodriguez,W.A.Offen,M.E.Palm-Espling,A.Pordea, M.R.Wormald,M.Mcdonough,G.J.Davies,B.G.Davis

Key ref:

M.Sharma et al. Cooperative bio-Metallic selectivity in a tailored pr enables creation of a c-C cross-Coupling heckase. To be published, .

Date:

24-Sep-15 Release date: 14-Sep-16

Protein chain

P29600  (SUBS_LEDLE) -  Subtilisin Savinase from Lederbergia lenta

Seq: 269 a.a.

Struc: 269 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.21.62 - subtilisin.
• Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.