PDBsum entry 4pfp

Motor protein

PDB id: 4pfp

Contents

Protein chains

760 a.a.

Ligands

ADP ×2

PGO

PO4 ×3

Metals

_MG ×2

Waters ×411

PDB id:

4pfp

Name:

Motor protein

Title:

Myosin vi motor domain in the pi release state (with pi) space group p21

Structure:

Unconventional myosin-vi. Chain: a, c. Fragment: residues 2-789. Engineered: yes

Source:

Sus scrofa. Pig. Organism_taxid: 9823. Gene: myo6. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9.

Resolution:

2.32Å R-factor: 0.191 R-free: 0.229

Authors:

T.Isabet,H.Benisty,P.Llinas,H.L.Sweeney,A.Houdusse

Key ref:

P.Llinas et al. (2015). How actin initiates the motor activity of Myosin. Dev Cell, 33, 401-412. PubMed id: 25936506 DOI: 10.1016/j.devcel.2015.03.025

Date:

30-Apr-14 Release date: 29-Apr-15

Protein chains

F1RQI7  (F1RQI7_PIG) -  Unconventional myosin-VI from Sus scrofa

Seq: 1266 a.a.

Struc: 760 a.a.

DOI no: 10.1016/j.devcel.2015.03.025

Dev Cell 33:401-412 (2015)

PubMed id: 25936506

How actin initiates the motor activity of Myosin.

P.Llinas, T.Isabet, L.Song, V.Ropars, B.Zong, H.Benisty, S.Sirigu, C.Morris, C.Kikuti, D.Safer, H.L.Sweeney, A.Houdusse.

ABSTRACT

Fundamental to cellular processes are directional movements driven by molecular motors. A common theme for these and other molecular machines driven by ATP is that controlled release of hydrolysis products is essential for using the chemical energy efficiently. Mechanochemical transduction by myosin motors on actin is coupled to unknown structural changes that result in the sequential release of inorganic phosphate (Pi) and MgADP. We present here a myosin structure possessing an actin-binding interface and a tunnel (back door) that creates an escape route for Pi with a minimal rotation of the myosin lever arm that drives movements. We propose that this state represents the beginning of the powerstroke on actin and that Pi translocation from the nucleotide pocket triggered by actin binding initiates myosin force generation. This elucidates how actin initiates force generation and movement and may represent a strategy common to many molecular machines.