PDBsum entry 4m7e

Transferase

PDB id: 4m7e

Contents

Protein chains

733 a.a.

175 a.a.

Ligands

NAG-NAG ×5

SO4 ×2

BLD ×2

NAG

PDB id:

4m7e

Name:

Transferase

Title:

Structural insight into bl-induced activation of the bri1-bak1 complex

Structure:

Protein brassinosteroid insensitive 1. Chain: a, b. Fragment: unp residues 24-785. Synonym: atbri1, brassinosteroid lrr receptor kinase. Engineered: yes. Brassinosteroid insensitive 1-associated receptor kinase 1. Chain: d, c. Fragment: unp residues 26-221. Synonym: atbak1, bri1-associated receptor kinase 1, protein

Source:

Arabidopsis thaliana. Mouse-ear cress,thale-cress. Organism_taxid: 3702. Gene: bri1, at4g39400, f23k16.30. Expression_system_cell: high five. Gene: bak1, elg, serk3, at4g33430, f17m5.190.

Resolution:

3.60Å R-factor: 0.223 R-free: 0.275

Authors:

J.Chai,Z.Han,Y.Sun

Key ref:

Y.Sun et al. Structural insight into bl-Induced activation of the bri1-Bak1 complex. To be published, .

Date:

12-Aug-13 Release date: 16-Oct-13

Protein chains

O22476  (BRI1_ARATH) -  Protein BRASSINOSTEROID INSENSITIVE 1 from Arabidopsis thaliana

Seq: 1196 a.a.

Struc: 733 a.a.

Protein chains

Q94F62  (BAK1_ARATH) -  BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 from Arabidopsis thaliana

Seq: 615 a.a.

Struc: 175 a.a.

Enzyme reactions

• Enzyme class 2: Chains A, B, D, C: E.C.2.7.10.1 - receptor protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] (Bound ligand (Het Group name = NAG) matches with 41.38% similarity) + ADP + H(+)

• Enzyme class 3: Chains A, B, D, C: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = NAG) matches with 41.38% similarity) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = NAG) matches with 41.38% similarity) + ADP + H(+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site