PDBsum entry 4fx3

Transferase/transferase inhibitor

PDB id: 4fx3

Contents

Protein chains

298 a.a.

257 a.a.

Ligands

60K ×2

DTT ×3

Waters ×154

PDB id:

4fx3

Name:

Transferase/transferase inhibitor

Title:

Crystal structure of the cdk2/cyclin a complex with oxindole inhibitor

Structure:

Cyclin-dependent kinase 2. Chain: a, c. Synonym: cell division protein kinase 2, p33 protein kinase. Engineered: yes. Cyclin-a2. Chain: b, d. Fragment: unp residues 175-432. Synonym: cyclin-a. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cdk2, cdkn2. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: high-five. Gene: ccn1, ccna, ccna2, cyclin a.

Resolution:

2.75Å R-factor: 0.192 R-free: 0.240

Authors:

Y.N.Kang,J.A.Stuckey

Key ref:

Y.N.Kang and j.a.stuckey Crystal structure of the cdk2/cyclin a complex with o inhibitor. To be published, .

Date:

02-Jul-12 Release date: 15-May-13

Protein chains

P24941  (CDK2_HUMAN) -  Cyclin-dependent kinase 2 from Homo sapiens

Seq: 298 a.a.

Struc: 298 a.a.

Protein chains

P20248  (CCNA2_HUMAN) -  Cyclin-A2 from Homo sapiens

Seq: 432 a.a.

Struc: 257 a.a.

Enzyme reactions

• Enzyme class: Chains A, C: E.C.2.7.11.22 - cyclin-dependent kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site