PDBsum entry 4din

Transferase/transport protein

PDB id

4din

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Contents

			Protein chains

					342 a.a.


					345 a.a.

			Ligands

					ATP

			Metals

					_MG ×2

PDB id:

4din

Links

Name:

Transferase/transport protein

Title:

Novel localization and quaternary structure of the pka ri beta holoenzyme

Structure:

Camp-dependent protein kinase catalytic subunit alpha. Chain: a. Synonym: pka c-alpha. Engineered: yes. Camp-dependent protein kinase type i-beta regulatory subunit. Chain: b. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: prkaca, pkaca. Expressed in: escherichia coli. Expression_system_taxid: 562. Homo sapiens. Human. Organism_taxid: 9606.

Resolution:

3.70Å

R-factor:

0.211

R-free:

0.293

Authors:

R.Ilouz,J.Bubis,J.Wu,Y.Y.Yim,M.S.Deal,A.P.Kornev,Y.Ma,D.K.Blumenthal, S.S.Taylor

Key ref:

R.Ilouz et al. (2012). Localization and quaternary structure of the PKA RIβ holoenzyme. Proc Natl Acad Sci U S A, 109, 12443-12448. PubMed id: 22797896

Date:

31-Jan-12

Release date:

04-Jul-12

PROCHECK

	Headers

	References

Protein chain

P05132 (KAPCA_MOUSE) - cAMP-dependent protein kinase catalytic subunit alpha from Mus musculus

Seq: Struc:					351 a.a. 342 a.a.*

Protein chain

P31321 (KAP1_HUMAN) - cAMP-dependent protein kinase type I-beta regulatory subunit from Homo sapiens

Seq: Struc:					381 a.a. 345 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class:

Chain A: E.C.2.7.11.11 - cAMP-dependent protein kinase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1.	L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
2.	L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] Bound ligand (Het Group name = ATP) corresponds exactly	+	ATP	=	O-phospho-L-seryl-[protein]	+	ADP	+	H(+)

L-threonyl-[protein] Bound ligand (Het Group name = ATP) corresponds exactly	+	ATP	=	O-phospho-L-threonyl-[protein]	+	ADP	+	H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Proc Natl Acad Sci U S A 109:12443-12448 (2012)
PubMed id: 22797896

Localization and quaternary structure of the PKA RIβ holoenzyme.
R.Ilouz, J.Bubis, J.Wu, Y.Y.Yim, M.S.Deal, A.P.Kornev, Y.Ma, D.K.Blumenthal, S.S.Taylor.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

22992589

S.S.Taylor, R.Ilouz, P.Zhang, and A.P.Kornev (2012).
Assembly of allosteric macromolecular switches: lessons from PKA.

Nat Rev Mol Cell Biol, 13, 646-658.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.