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PDBsum entry 4cn3
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Transcription/DNA
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PDB id
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4cn3
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References listed in PDB file
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Key reference
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Title
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Structural basis of natural promoter recognition by the retinoid X nuclear receptor.
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Authors
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J.Osz,
A.G.Mcewen,
P.Poussin-Courmontagne,
E.Moutier,
C.Birck,
I.Davidson,
D.Moras,
N.Rochel.
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Ref.
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Sci Rep, 2015,
5,
8216.
[DOI no: ]
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PubMed id
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Abstract
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Retinoid X receptors (RXRs) act as homodimers or heterodimerisation partners of
class II nuclear receptors. RXR homo- and heterodimers bind direct repeats of
the half-site (A/G)G(G/T)TCA separated by 1 nucleotide (DR1). We present a
structural characterization of RXR-DNA binding domain (DBD) homodimers on
several natural DR1s and an idealized symmetric DR1. Homodimers displayed
asymmetric binding, with critical high-affinity interactions accounting for the
3' positioning of RXR in heterodimers on DR1s. Differing half-site and spacer
DNA sequence induce changes in RXR-DBD homodimer conformation notably in the
dimerization interface such that natural DR1s are bound with higher affinity
than an idealized symmetric DR1. Subtle changes in the consensus DR1 DNA
sequence therefore specify binding affinity through altered RXR-DBD-DNA contacts
and changes in DBD conformation suggesting a general model whereby preferential
half-site recognition determines polarity of heterodimer binding to response
elements.
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