PDBsum entry 4qrx

Hydrolase

PDB id: 4qrx

Contents

Protein chains

292 a.a.

307 a.a.

Waters ×73

PDB id:

4qrx

Name:

Hydrolase

Title:

Crystal structure of pro-papain mutant at ph 4.0

Structure:

Pro-papain. Chain: a, c. Fragment: unp residues 27-345. Synonym: papaya proteinase i, ppi. Engineered: yes. Mutation: yes

Source:

Carica papaya. Mamon. Organism_taxid: 3649. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

3.14Å R-factor: 0.167 R-free: 0.254

Authors:

S.Dutta,D.Choudhury,S.Roy,S.Biswas

Key ref:

S.Dutta et al. Pro-Peptide regulates the substrate specificity and z activation process of papain: a structural and mechan insight. To be published, .

Date:

02-Jul-14 Release date: 05-Aug-15

Protein chain

P00784  (PAPA1_CARPA) -  Papain from Carica papaya

Seq: 345 a.a.

Struc: 292 a.a.*

Protein chain

P00784  (PAPA1_CARPA) -  Papain from Carica papaya

Seq: 345 a.a.

Struc: 307 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, C: E.C.3.4.22.2 - papain.
• Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, with preference for a residue bearing a large hydrophobic sidechain at the P2 position. Does not accept Val at P1'.