PDBsum entry 4qrg

Hydrolase

PDB id: 4qrg

Contents

Protein chains

300 a.a.

Metals

_CL ×2

_NA

Waters ×113

PDB id:

4qrg

Name:

Hydrolase

Title:

Crystal structure of i86l mutant of papain

Structure:

Papain. Chain: a, b. Fragment: unp residues 27-345. Synonym: papaya proteinase i, ppi. Engineered: yes. Mutation: yes

Source:

Carica papaya. Mamon. Organism_taxid: 3649. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.50Å R-factor: 0.162 R-free: 0.223

Authors:

S.Dutta,D.Choudhury,S.Roy,S.Biswas

Key ref:

S.Dutta et al. Pro-Peptide regulates the substrate specificity and z activation process of papain: a structural and mechan insight. To be published, .

Date:

01-Jul-14 Release date: 05-Aug-15

Protein chains

P00784  (PAPA1_CARPA) -  Papain from Carica papaya

Seq: 345 a.a.

Struc: 300 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.22.2 - papain.
• Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, with preference for a residue bearing a large hydrophobic sidechain at the P2 position. Does not accept Val at P1'.