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PDBsum entry 3zyj
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Cell adhesion
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PDB id
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3zyj
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References listed in PDB file
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Key reference
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Title
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Structural basis for cell surface patterning through netring-Ngl interactions.
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Authors
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E.Seiradake,
C.H.Coles,
P.V.Perestenko,
K.Harlos,
R.A.Mcilhinney,
A.R.Aricescu,
E.Y.Jones.
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Ref.
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Embo J, 2011,
30,
4479-4488.
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PubMed id
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Abstract
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Brain wiring depends on cells making highly localized and selective connections
through surface protein-protein interactions, including those between NetrinGs
and NetrinG ligands (NGLs). The NetrinGs are members of the structurally
uncharacterized netrin family. We present a comprehensive crystallographic
analysis comprising NetrinG1-NGL1 and NetrinG2-NGL2 complexes, unliganded
NetrinG2 and NGL3. Cognate NetrinG-NGL interactions depend on three
specificity-conferring NetrinG loops, clasped tightly by matching NGL surfaces.
We engineered these NGL surfaces to implant custom-made affinities for NetrinG1
and NetrinG2. In a cellular patterning assay, we demonstrate that
NetrinG-binding selectivity can direct the sorting of a mixed population of NGLs
into discrete cell surface subdomains. These results provide a molecular model
for selectivity-based patterning in a neuronal recognition system, dysregulation
of which is associated with severe neuropsychological disorders.
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