PDBsum entry 3qep

Transferase/DNA

PDB id

3qep

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Contents

			Protein chain

					901 a.a.

			DNA/RNA

			Ligands

					TTP

			Metals

					_CA ×7

			Waters ×1239

PDB id:

3qep

Links

Name:

Transferase/DNA

Title:

Rb69 DNA polymerase (l561a/s565g/y567a) ternary complex with dttp opposite difluorotoluene nucleoside

Structure:

DNA polymerase. Chain: a. Synonym: gp43. Engineered: yes. Mutation: yes. DNA (5'-d( Tp Cp Gp (Dft) p Gp Tp Ap Ap Gp Cp Ap Gp Tp Cp Cp Gp Cp G)-3'). Chain: t. Engineered: yes.

Source:

Enterobacteria phage rb69. Bacteriophage rb69. Organism_taxid: 12353. Gene: 43. Expressed in: escherichia coli. Expression_system_taxid: 511693. Synthetic: yes. Organism_taxid: 32630. Organism_taxid: 32630

Resolution:

1.80Å

R-factor:

0.169

R-free:

0.204

Authors:

S.Xia,W.H.Konigsberg,J.Wang

Key ref:

S.Xia et al. (2011). Hydrogen-bonding capability of a templating difluorotoluene nucleotide residue in an RB69 DNA polymerase ternary complex. J Am Chem Soc, 133, 10003-10005. PubMed id: 21667997

Date:

20-Jan-11

Release date:

31-Aug-11

PROCHECK

	Headers

	References

Protein chain

Q38087 (DPOL_BPR69) - DNA-directed DNA polymerase from Escherichia phage RB69

Seq: Struc:

Seq: Struc:					903 a.a. 901 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

DNA/RNA chains

		T-C-G-DFT-G-T-A-A-G-C-A-G-T-C-C-G-C-G 18 bases
		G-C-G-G-A-C-T-G-C-T-T-A-DOC 13 bases



		Enzyme reactions

Enzyme class 1:

E.C.2.7.7.7 - DNA-directed Dna polymerase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

DNA(n)	+	2'-deoxyribonucleoside 5'-triphosphate	=	DNA(n+1)	+	diphosphate

Enzyme class 2:

E.C.3.1.11.- - ?????

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

	J Am Chem Soc 133:10003-10005 (2011)
PubMed id: 21667997

Hydrogen-bonding capability of a templating difluorotoluene nucleotide residue in an RB69 DNA polymerase ternary complex.
S.Xia, W.H.Konigsberg, J.Wang.

ABSTRACT

No abstract given.