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PDBsum entry 3pml
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Lyase,transferase/DNA
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PDB id
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3pml
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Contents |
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* Residue conservation analysis
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PDB id:
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Lyase,transferase/DNA
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Title:
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Crystal structure of a polymerase lambda variant with a dgtp analog opposite a templating t
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Structure:
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DNA polymerase lambda. Chain: a, b. Fragment: unp residues 242-575. Synonym: pol lambda, DNA polymerase beta-2, pol beta2, DNA polymerase kappa. Engineered: yes. 5'-d( Cp Gp Gp Cp Tp Gp Tp Ap Cp Tp G)-3'. Chain: c, e. Engineered: yes.
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Strain: 562. Gene: poll. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic: yes
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Resolution:
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2.60Å
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R-factor:
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0.233
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R-free:
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0.271
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Authors:
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K.Bebenek,L.C.Pedersen,T.A.Kunkel
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Key ref:
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K.Bebenek
et al.
(2011).
Replication infidelity via a mismatch with Watson-Crick geometry.
Proc Natl Acad Sci U S A,
108,
1862-1867.
PubMed id:
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Date:
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17-Nov-10
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Release date:
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26-Jan-11
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PROCHECK
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Headers
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References
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Q9UGP5
(DPOLL_HUMAN) -
DNA polymerase lambda from Homo sapiens
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Seq:
Struc:
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575 a.a.
322 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 3 residue positions (black
crosses)
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C-G-G-C-T-G-T-A-C-T-G
11 bases
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G-C-C-G
4 bases
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C-G-G-C-T-G-T-A-C-T-G
11 bases
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C-A-G-T-A-C
6 bases
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G-C-C-G
4 bases
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C-A-G-T-A-C
6 bases
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Enzyme class 2:
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E.C.2.7.7.7
- DNA-directed Dna polymerase.
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Reaction:
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DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
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DNA(n)
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+
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2'-deoxyribonucleoside 5'-triphosphate
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=
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DNA(n+1)
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+
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diphosphate
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Enzyme class 3:
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E.C.4.2.99.-
- ?????
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Proc Natl Acad Sci U S A
108:1862-1867
(2011)
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PubMed id:
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Replication infidelity via a mismatch with Watson-Crick geometry.
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K.Bebenek,
L.C.Pedersen,
T.A.Kunkel.
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ABSTRACT
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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N.Demeshkina,
L.Jenner,
E.Westhof,
M.Yusupov,
and
G.Yusupova
(2012).
A new understanding of the decoding principle on the ribosome.
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Nature,
484,
256-259.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
