PDBsum entry 3oks

Transferase

PDB id: 3oks

Contents

Protein chains

444 a.a. *

Ligands

EDO ×9

FMT ×12

Metals

_MG

Waters ×1545

* Residue conservation analysis

PDB id:

3oks

Name:

Transferase

Title:

Crystal structure of 4-aminobutyrate transaminase from mycobacterium smegmatis

Structure:

4-aminobutyrate transaminase. Chain: a, b, c, d. Engineered: yes

Source:

Mycobacterium smegmatis. Organism_taxid: 246196. Strain: atcc 700084/mc(2)155. Gene: gabt, msmeg_2959. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.80Å R-factor: 0.128 R-free: 0.159

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

L.Baugh et al. (2015). Increasing the structural coverage of tuberculosis drug targets. Tuberculosis (edinb), 95, 142-148. PubMed id: 25613812 DOI: 10.1016/j.tube.2014.12.003

Date:

25-Aug-10 Release date: 27-Oct-10

Protein chains

A0QWJ0  (A0QWJ0_MYCS2) -  4-aminobutyrate aminotransferase from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: 446 a.a.

Struc: 444 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: E.C.2.6.1.19 - 4-aminobutyrate--2-oxoglutarate transaminase.
• Reaction: 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate

4-aminobutanoate (Bound ligand (Het Group name = FMT) matches with 42.86% similarity) + 2-oxoglutarate (Bound ligand (Het Group name = EDO) matches with 40.00% similarity) = succinate semialdehyde + L-glutamate

• Cofactor: Pyridoxal 5'-phosphate
• Enzyme class 2: E.C.2.6.1.22 - (S)-3-amino-2-methylpropionate transaminase.

Pathway:

• Reaction: (S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3- oxopropanoate + L-glutamate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.tube.2014.12.003

Tuberculosis (edinb) 95:142-148 (2015)

PubMed id: 25613812

Increasing the structural coverage of tuberculosis drug targets.

L.Baugh, I.Phan, D.W.Begley, M.C.Clifton, B.Armour, D.M.Dranow, B.M.Taylor, M.M.Muruthi, J.Abendroth, J.W.Fairman, D.Fox, S.H.Dieterich, B.L.Staker, A.S.Gardberg, R.Choi, S.N.Hewitt, A.J.Napuli, J.Myers, L.K.Barrett, Y.Zhang, M.Ferrell, E.Mundt, K.Thompkins, N.Tran, S.Lyons-Abbott, A.Abramov, A.Sekar, D.Serbzhinskiy, D.Lorimer, G.W.Buchko, R.Stacy, L.J.Stewart, T.E.Edwards, W.C.Van Voorhis, P.J.Myler.

ABSTRACT

No abstract given.