PDBsum entry 3knb

Structural protein/structural protein

PDB id

3knb

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Contents

			Protein chains

					98 a.a. *


					96 a.a. *

			Ligands

					SO4

			Waters ×266

* Residue conservation analysis

PDB id:

3knb

Links

Name:

Structural protein/structural protein

Title:

Crystal structure of the titin c-terminus in complex with obscurin- like 1

Structure:

Titin. Chain: a. Fragment: titin, c-terminal domain m10, resideus 34253-34350. Synonym: connectin, rhabdomyosarcoma antigen mu-rms-40.14. Engineered: yes. Obscurin-like protein 1. Chain: b. Fragment: obscurin-like 1, n-terminal domain, residues 1-105. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ttn. Expressed in: escherichia coli. Expression_system_taxid: 511693. Gene: kiaa0657, obsl1.

Resolution:

1.40Å

R-factor:

0.165

R-free:

0.195

Authors:

F.Sauer,J.Vahokoski,M.Wilmanns

Key ref:

F.Sauer et al. (2010). Molecular basis of the head-to-tail assembly of giant muscle proteins obscurin-like 1 and titin. Embo Rep, 11, 534-540. PubMed id: 20489725

Date:

12-Nov-09

Release date:

01-Dec-09

Supersedes:

3irg

PROCHECK

	Headers

	References

Protein chain

Q8WZ42 (TITIN_HUMAN) - Titin from Homo sapiens

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Seq: Struc:					34350 a.a. 98 a.a.*

Protein chain

O75147 (OBSL1_HUMAN) - Obscurin-like protein 1 from Homo sapiens

Seq: Struc:

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Seq: Struc:					1896 a.a. 96 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class:

Chain A: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1.	L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
2.	L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein]	+	ATP	=	O-phospho-L-seryl-[protein]	+	ADP	+	H(+)

L-threonyl-[protein]	+	ATP	=	O-phospho-L-threonyl-[protein]	+	ADP	+	H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Embo Rep 11:534-540 (2010)
PubMed id: 20489725

Molecular basis of the head-to-tail assembly of giant muscle proteins obscurin-like 1 and titin.
F.Sauer, J.Vahokoski, Y.H.Song, M.Wilmanns.

ABSTRACT

Large filament proteins in muscle sarcomeres comprise many immunoglobulin-like domains that provide a molecular platform for self-assembly and interactions with heterologous protein partners. We have unravelled the molecular basis for the head-to-tail interaction of the carboxyl terminus of titin and the amino-terminus of obscurin-like-1 by X-ray crystallography. The binary complex is formed by a parallel intermolecular beta-sheet that presents a novel immunoglobulin-like domain-mediated assembly mechanism in muscle filament proteins. Complementary binding data show that the assembly is entropy-driven rather than dominated data by specific polar interactions. The assembly observed leads to a V-shaped zipper-like arrangement of the two filament proteins.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21190822

M.Gautel (2011).
The sarcomeric cytoskeleton: who picks up the strain?

Curr Opin Cell Biol, 23, 39-46.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.