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PDBsum entry 3gdx
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Transferase/DNA
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PDB id
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3gdx
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References listed in PDB file
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Key reference
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Title
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Alpha,Beta-Difluoromethylene deoxynucleoside 5'-Triphosphates: a convenient synthesis of useful probes for DNA polymerase beta structure and function.
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Authors
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T.G.Upton,
B.A.Kashemirov,
C.E.Mckenna,
M.F.Goodman,
G.K.Prakash,
R.Kultyshev,
V.K.Batra,
D.D.Shock,
L.C.Pedersen,
W.A.Beard,
S.H.Wilson.
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Ref.
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Org Lett, 2009,
11,
1883-1886.
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PubMed id
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Abstract
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Alpha,beta-difluoromethylene deoxynucleoside 5'-triphosphates (dNTPs, N = A or
C) are advantageously obtained via phosphorylation of corresponding dNDP
analogues using catalytic ATP, PEP, nucleoside diphosphate kinase, and pyruvate
kinase. DNA pol beta K(d) values for the alpha,beta-CF(2) and unmodified dNTPs,
alpha,beta-NH dUTP, and the alpha,beta-CH(2) analogues of dATP and dGTP are
discussed in relation to the conformations of alpha,beta-CF(2) dTTP versus
alpha,beta-NH dUTP bound into the enzyme active site.
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