PDBsum entry 3usv

Hydrolase

PDB id: 3usv

Contents

Protein chains

308 a.a.

PDB id:

3usv

Name:

Hydrolase

Title:

Structure of the precursor of a thermostable variant of papain at 3.8 a resolution from a crystal soaked at ph 4

Structure:

Papain. Chain: a, c. Fragment: unp residues 27-345. Synonym: papaya proteinase i, ppi. Engineered: yes. Mutation: yes

Source:

Carica papaya. Mamon. Organism_taxid: 3649. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

3.80Å R-factor: 0.358 R-free: 0.408

Authors:

S.Roy,D.Choudhury,S.Biswas,J.K.Dattagupta

Key ref:

S.Roy et al. Crystallographic analysis of pro-Papain variant eluci the structural basis of the step-Wise activation mech of the zymogen. To be published, .

Date:

24-Nov-11 Release date: 28-Nov-12

Protein chains

P00784  (PAPA1_CARPA) -  Papain from Carica papaya

Seq: 345 a.a.

Struc: 308 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.22.2 - papain.
• Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, with preference for a residue bearing a large hydrophobic sidechain at the P2 position. Does not accept Val at P1'.