PDBsum entry 3tnx

Hydrolase

PDB id: 3tnx

Contents

Protein chains

310 a.a.

Metals

_CL

Waters ×133

PDB id:

3tnx

Name:

Hydrolase

Title:

Structure of the precursor of a thermostable variant of papain at 2.6 angstroem resolution

Structure:

Papain. Chain: a, c. Fragment: unp residues 27-345. Synonym: papaya proteinase i, ppi. Engineered: yes. Mutation: yes

Source:

Carica papaya. Mamon. Organism_taxid: 3649. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.62Å R-factor: 0.186 R-free: 0.236

Authors:

S.Roy,D.Choudhury,J.K.Dattagupta,S.Biswas

Key ref:

S.Roy et al. (2012). The structure of a thermostable mutant of pro-papain reveals its activation mechanism. Acta Crystallogr D Biol Crystallogr, 68, 1591-1603. PubMed id: 23151624

Date:

02-Sep-11 Release date: 12-Sep-12

Protein chains

P00784  (PAPA1_CARPA) -  Papain from Carica papaya

Seq: 345 a.a.

Struc: 310 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.22.2 - papain.
• Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, with preference for a residue bearing a large hydrophobic sidechain at the P2 position. Does not accept Val at P1'.

Acta Crystallogr D Biol Crystallogr 68:1591-1603 (2012)

PubMed id: 23151624

The structure of a thermostable mutant of pro-papain reveals its activation mechanism.

S.Roy, D.Choudhury, P.Aich, J.K.Dattagupta, S.Biswas.

ABSTRACT

No abstract given.