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PDBsum entry 2z7c
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DNA binding protein,RNA binding protein
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PDB id
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2z7c
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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DNA binding protein,RNA binding protein
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Title:
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Crystal structure of chromatin protein alba from hyperthermophilic archaeon pyrococcus horikoshii
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Structure:
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DNA/RNA-binding protein alba. Chain: a, b, c, d. Engineered: yes
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Source:
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Pyrococcus horikoshii. Organism_taxid: 53953. Strain: ot3. Gene: phs053. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.80Å
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R-factor:
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0.230
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R-free:
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0.318
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Authors:
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K.Hada,T.Nakashima,T.Osawa,H.Shimada,Y.Kakuta,M.Kimura
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Key ref:
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K.Hada
et al.
(2008).
Crystal structure and functional analysis of an archaeal chromatin protein Alba from the hyperthermophilic archaeon Pyrococcus horikoshii OT3.
Biosci Biotechnol Biochem,
72,
749-758.
PubMed id:
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Date:
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17-Aug-07
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Release date:
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05-Aug-08
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PROCHECK
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Headers
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References
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O74101
(ALBA_PYRHO) -
DNA/RNA-binding protein Alba from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
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Seq:
Struc:
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93 a.a.
89 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Biosci Biotechnol Biochem
72:749-758
(2008)
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PubMed id:
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Crystal structure and functional analysis of an archaeal chromatin protein Alba from the hyperthermophilic archaeon Pyrococcus horikoshii OT3.
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K.Hada,
T.Nakashima,
T.Osawa,
H.Shimada,
Y.Kakuta,
M.Kimura.
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ABSTRACT
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The crystal structure of the Alba protein (PhoAlba) from a hyperthermophilic
archaeon, Pyrococcus horikoshii OT3, was determined at a resolution of 2.8 A.
PhoAlba structurally belongs to the alpha/beta proteins and is similar not only
to archaeal homologues but also to RNA-binding proteins, including the
C-terminal half of initiation factor 3 (IF3-C) from Bacillus stearothermophilus,
an Esherichia coli protein implicated in cell division (Yhhp), and an
Arabidopsis protein of unknown function. We found by gel shift assay that
PhoAlba interacts with both ribonuclease P (RNase P) RNA (PhopRNA) and
precursor-tRNA(Tyr) (pre-tRNA(Tyr)) in P. horikoshii. However, the addition of
PhoAlba to reconstituted particles composed of PhopRNA and four or five protein
subunits had little influence on either the pre-tRNA processing activity or the
optimum temperature for the processing activity. These results suggest that
PhoAlba contributes little to the catalytic activity of P. horikoshii RNase P.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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I.M.Cho,
L.B.Lai,
D.Susanti,
B.Mukhopadhyay,
and
V.Gopalan
(2010).
Ribosomal protein L7Ae is a subunit of archaeal RNase P.
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Proc Natl Acad Sci U S A,
107,
14573-14578.
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N.Jarrous,
and
V.Gopalan
(2010).
Archaeal/eukaryal RNase P: subunits, functions and RNA diversification.
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Nucleic Acids Res,
38,
7885-7894.
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S.C.Dillon,
and
C.J.Dorman
(2010).
Bacterial nucleoid-associated proteins, nucleoid structure and gene expression.
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Nat Rev Microbiol,
8,
185-195.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
