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PDBsum entry 2z7c
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DNA binding protein,RNA binding protein
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PDB id
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2z7c
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References listed in PDB file
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Key reference
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Title
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Crystal structure and functional analysis of an archaeal chromatin protein alba from the hyperthermophilic archaeon pyrococcus horikoshii ot3.
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Authors
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K.Hada,
T.Nakashima,
T.Osawa,
H.Shimada,
Y.Kakuta,
M.Kimura.
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Ref.
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Biosci Biotechnol Biochem, 2008,
72,
749-758.
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PubMed id
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Abstract
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The crystal structure of the Alba protein (PhoAlba) from a hyperthermophilic
archaeon, Pyrococcus horikoshii OT3, was determined at a resolution of 2.8 A.
PhoAlba structurally belongs to the alpha/beta proteins and is similar not only
to archaeal homologues but also to RNA-binding proteins, including the
C-terminal half of initiation factor 3 (IF3-C) from Bacillus stearothermophilus,
an Esherichia coli protein implicated in cell division (Yhhp), and an
Arabidopsis protein of unknown function. We found by gel shift assay that
PhoAlba interacts with both ribonuclease P (RNase P) RNA (PhopRNA) and
precursor-tRNA(Tyr) (pre-tRNA(Tyr)) in P. horikoshii. However, the addition of
PhoAlba to reconstituted particles composed of PhopRNA and four or five protein
subunits had little influence on either the pre-tRNA processing activity or the
optimum temperature for the processing activity. These results suggest that
PhoAlba contributes little to the catalytic activity of P. horikoshii RNase P.
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