PDBsum entry 2y5f

Blood clotting

PDB id: 2y5f

Contents

Protein chains

234 a.a.

54 a.a.

Ligands

XWG

Metals

_NA ×2

Waters ×399

PDB id:

2y5f

Name:

Blood clotting

Title:

Factor xa - cation inhibitor complex

Structure:

Activated factor xa heavy chain. Chain: a. Fragment: residues 235-468. Synonym: coagulation factor xa. Engineered: yes. Mutation: yes. Factor x light chain. Chain: l. Fragment: residues 127-180.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

1.29Å R-factor: 0.140 R-free: 0.175

Authors:

D.W.Banner,L.M.Salonen,M.C.Holland,W.Haap,J.Benz,F.Diederich

Key ref:

L.M.Salonen et al. (2012). Molecular recognition at the active site of factor Xa: cation-π interactions, stacking on planar peptide surfaces, and replacement of structural water. Chemistry, 18, 213-222. PubMed id: 22162109

Date:

13-Jan-11 Release date: 28-Dec-11

Protein chain

P00742  (FA10_HUMAN) -  Coagulation factor X from Homo sapiens

Seq: 488 a.a.

Struc: 234 a.a.*

Protein chain

P00742  (FA10_HUMAN) -  Coagulation factor X from Homo sapiens

Seq: 488 a.a.

Struc: 54 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, L: E.C.3.4.21.6 - coagulation factor Xa.
• Reaction: Preferential cleavage: Arg-|-Thr and then Arg-|-Ile bonds in prothrombin to form thrombin.

Chemistry 18:213-222 (2012)

PubMed id: 22162109

Molecular recognition at the active site of factor Xa: cation-π interactions, stacking on planar peptide surfaces, and replacement of structural water.

L.M.Salonen, M.C.Holland, P.S.Kaib, W.Haap, J.Benz, J.L.Mary, O.Kuster, W.B.Schweizer, D.W.Banner, F.Diederich.

ABSTRACT

No abstract given.