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487 a.a.
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467 a.a.
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182 a.a.
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83 a.a.
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25 a.a.
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* Residue conservation analysis
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PDB id:
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Hydrolase
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Title:
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Low resolution structures of bovine mitochondrial f1-atpase during controlled dehydration: hydration state 5.
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Structure:
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Atp synthase subunit alpha heart isoform, mitochondrial. Chain: a, b, c. Synonym: f1-atpase alpha subunit. Atp synthase subunit beta, mitochondrial. Chain: d, e, f. Synonym: f1-atpase beta subunit. Atp synthase subunit gamma, mitochondrial. Chain: g. Synonym: f1-atpase gamma subunit.
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Source:
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Bos taurus. Bovine. Organism_taxid: 9913. Organ: heart. Tissue: muscle. Organelle: mitochondria. Organelle: mitochonria. Organelle: mitochonria
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Resolution:
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Authors:
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J.Sanchez-Weatherby,F.Felisaz,A.Gobbo,J.Huet,R.B.G.Ravelli, M.W.Bowler,F.Cipriani
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Key ref:
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J.Sanchez-Weatherby
et al.
(2009).
Improving diffraction by humidity control: a novel device compatible with X-ray beamlines.
Acta Crystallogr D Biol Crystallogr,
65,
1237-1246.
PubMed id:
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Date:
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18-Dec-08
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Release date:
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27-Oct-09
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PROCHECK
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Headers
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References
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P19483
(ATPA_BOVIN) -
ATP synthase subunit alpha, mitochondrial from Bos taurus
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Seq:
Struc:
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553 a.a.
487 a.a.
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P00829
(ATPB_BOVIN) -
ATP synthase subunit beta, mitochondrial from Bos taurus
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Seq:
Struc:
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528 a.a.
467 a.a.
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P05631
(ATPG_BOVIN) -
ATP synthase subunit gamma, mitochondrial from Bos taurus
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Seq:
Struc:
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298 a.a.
182 a.a.
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Enzyme class 2:
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Chains A, B, C, G, H, I:
E.C.3.6.3.14
- Transferred entry: 7.1.2.2.
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Reaction:
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ATP + H2O + H+(In) = ADP + phosphate + H+(Out)
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ATP
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+
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H(2)O
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+
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H(+)(In)
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=
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ADP
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+
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phosphate
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+
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H(+)(Out)
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Enzyme class 3:
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Chains D, E, F:
E.C.7.1.2.2
- H(+)-transporting two-sector ATPase.
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Reaction:
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ATP + H2O + 4 H+(in) = ADP + phosphate + 5 H+(out)
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ATP
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+
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H2O
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+
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4
×
H(+)(in)
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=
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ADP
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+
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phosphate
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+
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5
×
H(+)(out)
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Acta Crystallogr D Biol Crystallogr
65:1237-1246
(2009)
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PubMed id:
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Improving diffraction by humidity control: a novel device compatible with X-ray beamlines.
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J.Sanchez-Weatherby,
M.W.Bowler,
J.Huet,
A.Gobbo,
F.Felisaz,
B.Lavault,
R.Moya,
J.Kadlec,
R.B.Ravelli,
F.Cipriani.
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ABSTRACT
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Dehydration of protein crystals is rarely used, despite being a
post-crystallization method that is useful for the improvement of crystal
diffraction properties, as it is difficult to reproduce and monitor. A novel
device for hydration control of macromolecular crystals in a standard
data-collection environment has been developed. The device delivers an air
stream of precise relative humidity that can be used to alter the amount of
water in macromolecular crystals. The device can be rapidly installed and is
fully compatible with most standard synchrotron X-ray beamlines. Samples are
mounted in cryoloops and the progress of dehydration can be monitored both
optically and by the acquisition of diffraction images. Once the optimal
hydration level has been obtained, cryocooling is easy to achieve by hand or by
using a sample changer. The device has been thoroughly tested on several ESRF
beamlines and is available to users.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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C.Rajendran,
F.S.Dworkowski,
M.Wang,
and
C.Schulze-Briese
(2011).
Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector.
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J Synchrotron Radiat,
18,
318-328.
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J.Kadlec,
E.Hallacli,
M.Lipp,
H.Holz,
J.Sanchez-Weatherby,
S.Cusack,
and
A.Akhtar
(2011).
Structural basis for MOF and MSL3 recruitment into the dosage compensation complex by MSL1.
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Nat Struct Mol Biol,
18,
142-149.
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PDB codes:
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N.J.Hu,
S.Iwata,
A.D.Cameron,
and
D.Drew
(2011).
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.
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Nature,
478,
408-411.
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PDB codes:
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J.Gabadinho,
A.Beteva,
M.Guijarro,
V.Rey-Bakaikoa,
D.Spruce,
M.W.Bowler,
S.Brockhauser,
D.Flot,
E.J.Gordon,
D.R.Hall,
B.Lavault,
A.A.McCarthy,
J.McCarthy,
E.Mitchell,
S.Monaco,
C.Mueller-Dieckmann,
D.Nurizzo,
R.B.Ravelli,
X.Thibault,
M.A.Walsh,
G.A.Leonard,
and
S.M.McSweeney
(2010).
MxCuBE: a synchrotron beamline control environment customized for macromolecular crystallography experiments.
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J Synchrotron Radiat,
17,
700-707.
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M.W.Bowler,
M.Guijarro,
S.Petitdemange,
I.Baker,
O.Svensson,
M.Burghammer,
C.Mueller-Dieckmann,
E.J.Gordon,
D.Flot,
S.M.McSweeney,
and
G.A.Leonard
(2010).
Diffraction cartography: applying microbeams to macromolecular crystallography sample evaluation and data collection.
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Acta Crystallogr D Biol Crystallogr,
66,
855-864.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
