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PDBsum entry 2w6j
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Contents |
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487 a.a.
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467 a.a.
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182 a.a.
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83 a.a.
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25 a.a.
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References listed in PDB file
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Key reference
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Title
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Improving diffraction by humidity control: a novel device compatible with X-Ray beamlines.
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Authors
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J.Sanchez-Weatherby,
M.W.Bowler,
J.Huet,
A.Gobbo,
F.Felisaz,
B.Lavault,
R.Moya,
J.Kadlec,
R.B.Ravelli,
F.Cipriani.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2009,
65,
1237-1246.
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PubMed id
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Abstract
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Dehydration of protein crystals is rarely used, despite being a
post-crystallization method that is useful for the improvement of crystal
diffraction properties, as it is difficult to reproduce and monitor. A novel
device for hydration control of macromolecular crystals in a standard
data-collection environment has been developed. The device delivers an air
stream of precise relative humidity that can be used to alter the amount of
water in macromolecular crystals. The device can be rapidly installed and is
fully compatible with most standard synchrotron X-ray beamlines. Samples are
mounted in cryoloops and the progress of dehydration can be monitored both
optically and by the acquisition of diffraction images. Once the optimal
hydration level has been obtained, cryocooling is easy to achieve by hand or by
using a sample changer. The device has been thoroughly tested on several ESRF
beamlines and is available to users.
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